Therapeutic Effect And Mechanism Of Se-Se MSN In Pancreatitis

Pancreatitis is a serious disease of pancreas caused by trypsin digestion.Pancreatitis usually causes acinar cell atrophy,pancreatic tissue necrosis,and even systemic inflammatory response and multiple organ failure.The current treatment of pancreatitis has some drawbacks,and the mortality and recurrence rate of pancreatitis have not been improved.Therefore,it is an urgent problem to develop a new treatment for pancreatitis.Oxidative stress is an important factor in pancreatitis,ROS-induced oxidative stress can activate NF-κB signaling pathway,promoting the occurrence and development of pancreatitis.Se-Se MSN developed by us and South China University of Technology is a biodegradable particle,which can respond to excessive ROS in the microenvironment and reduce the level of oxidative stress,has a good therapeutic effect on acute sepsis,but whether it has a therapeutic effect on pancreatitis is still unclear.Therefore,this study will explore whether Se-Se MSN has therapeutic effect on pancreatitis and its possible mechanism.We established a model of chronic pancreatitis in C57/BL6 mice,and by H&E,Masson,amylase/CK19 immunofluorescence staining,α-SMA immunohistochemical staining and serum levels of amylase and lipase to evaluate the therapeutic effect of Se-Se MSN in chronic pancreatitis.We established a model of acute pancreatitis in SD rat,and by comparing the survival rate,survival time,degree of pancreatic edema and other indicators to evaluate the therapeutic effect of Se-Se MSN in acute pancreatitis.Finally,we measured the level of oxidative stress and the expression of NF-κB signaling pathway to elucidate the mechanism of Se-Se MSN in the treatment of pancreatitis.The results show that Se-Se MSN can reduce the severity of acinar duct metaplasia(ADM)and fibrosis,and increase serum levels of amylase and lipase in chronic pancreatitis,which indicates that Se-Se MSN has therapeutic effect on chronic pancreatitis in vivo;Se-Se MSN can reduce the formation of vacuolation,increase the expression of amylase and reduce the expression of CK19 in TGF-β1-induced primary acinar cells,which indicates that Se-Se MSN has therapeutic effect on chronic pancreatitis in vitro;Se-Se MSN can reduce the degree of pancreatic tissue damage,improve the survival rate of rats,prolong the survival time of rats,and reduce serum levels of amylase and lipase in acute pancreatitis,which indicates that Se-Se MSN has therapeutic effect on acute pancreatitis;Se-Se MSN can reduce the expression of MDA,increase the expression of SOD and reduce the expression of p-NF-κB in chronic and acute pancreatitis,which indicates that Se-Se MSN can inhibit NF-κB signaling pathway by decreasing the level of ROS to treat pancreatitis.