The Anti-tumor Biological Mechanism Of NuMA Antibody In Small Cell Lung Cancer

Background: Small cell lung cancer(SCLC)is a neuroendocrine tumor with high aggressiveness and poor prognosis.The effect of initial therapy is obvious,but it is prone to relapse and drug resistance.Most patients were already in the extensive stage of the tumor and accompanied by distal diffusion when they were diagnosed as SCLC.SCLC is strongly related to smoking.Tumor mutation burden(TMB)and the probability of somatic mutations were increased by tobacco carcinogens.Therefore,the tumor immune microenvironment has become the focus of an exciting area under investigation.Abnormal cell division is the main manifestation of SCLC.The spindle is a special organelle of cell division.If its physiological function cannot be controlled,leading to the excessive accumulation in the cells,so that the sister chromatids cannot be distributed evenly.The cells proliferate abnormally,which will eventually lead to tumors.The microtubule spindle antibody(MSA)is an autoantibody produced by the excessive proliferation of the spindle to stimulate the immune function of the body.It is closely related to the occurrence and development of SCLC.The nuclear mitotic apparatus protein(Nu MA)is one of the most important proteins in the process of mitosis,and its antibody has therefore become one of the most representative proteins in MSA.Objective: This study aims to clarify the effect of MSA on the biological functions of NCI-H446 small cell lung cancer cells,including morphology,proliferation,adhesion,apoptosis,migration and invasion,exploring MSA to provide new ideas on the pathogenesis and treatment of SCLC.Methods:(1)Using different concentrations of MSA to act on NCI-H446 small cell lung cancer cells to observe the difference in cell morphology.(2)Using CCK-8proliferation experiment to detect the effect of MSA on the in vitro proliferation of NCI-H446 small cell lung cancer cells.(3)The Annexin V-FITC/PI double staining method was used to detect the apoptosis effect of MSA on NCI-H446 small cell lung cancer cells.(4)Exploring the cell migration ability through the scratch test and cell scratch healing rate.(5)Transwell migration experiment was used to verify the effect of MSA on the migration of NCI-H446 small cell lung cancer cells.(6)Using the matrigel transwell invasion experiment to explore the effect of MSA on the invasion of NCI-H446 small cell lung cancer cells.Results:(1)NCI-H446 small cell lung cancer cells were treated with different concentrations of MSA,and no significant changes in cell morphology were seen.(2)After NCI-H446 cells were treated with different concentrations of MSA,no significant statistical difference was showed at 24h(P>0.05).Starting at 48 h,compared with the control group(MSA concentration of 0μg/L),the proliferation of NCI-H446 cells in the experimental group who was treated with MSA(concentrations of 0.5μg/L,1μg/L,2μg/L,4μg/L,8μg/L)was significantly inhibited(P<0.05),and did not show a concentration-dependent.(3)No effect of MSA on NCI-H446 small cell lung cancer cells adhesion was found by the treatment of cell adhesion experiment.(4)After using Annexin V-FITC/PI double-staining cell apoptosis experiment,it was found that the apoptosis rate of small cell lung cancer cells in the MSA treatment group was(35.09±7.76)%,which was significantly higher than that of the control group(17.34±3.07)%,the difference was statistically significant(P<0.05).(5)Both the scratch healing test and the transwell migration test showed that MSA has an inhibitory effect on the migration of NCI-H446 small cell lung cancer cells.(6)Through the matrigel invasion experiment,it was found that MSA inhibited the invasion of NCIH446 cells.Conclusion: This study investigated the clinical significance of MSA detection in SCLC disease and its effect on the biological behavior of NCI-H446 small cell lung cancer cells from the clinical and cellular level.This study found that MSA did not affect the morphology and attachment ability of NCI-H446 small cell lung cancer cells,but it significantly inhibited the proliferation,migration and invasion of NCI-H446 cells,and promoted their apoptosis.Provide new ideas for the research and treatment of SCLC pathogenesis.