TMEM106B Affects The Proliferation And Invasion Of Malignant Meningioma Cells Through PI3K/AKT Signaling Pathway

Objective:To investigate the impression of TMEM106 B on the abilities of proliferation,migration and invasion of malignant meningioma cells,and to reveal the relationship between TMEM106 B with the activation of PI3 K / Akt signaling pathway.Methods:1.Malignant meningiomas IOMM-Lee cell line and CH157 cell line were infected by lentivirus for the establishment of the cells lines that TMEM106 B down-regulated or up-regulated.Both cell lines were divided into five groups: blank group(Blank),negative control of silence group(NC-sh),silent TMEM106 B group(TMEM106B-sh),negative control of overexpression group(NC-ox),overexpression of TMEM106 B group(TMEM106B-ox).Real-time fluorescent quantitative PCR(RT-q PCR)and Western blot assay were used for detecting of the expression levels of TMEM106 B m RNA and protein in different group of cell lines.2.CCK8 and MTT assay were used for the evaluation of cell proliferation in different groups,the changes of cell cycle and apoptosis were detected by flow cytometry.3.Cell migration and invasion of each group were observed by cell scratch assay and Transwell assay,respectively.4.Western blot was performed for the detection of the protein expression levels of TMEM106 B,PI3K,p-PI3 K,AKT,p-AKT in different groups of both cell lines.5.SPSS 22.0 statistical software was used to analyze the data,all data were based on the mean of three repeated experiments ± standard deviation(SD).The difference between the two groups was compared by t-test,and the data between the two groups was compared by ANOVA.P < 0.05 was considered to be statistically significant.Results:1.The expression level of TMEM106 B m RNA in each group of cell lines was detected by RT-q PCR: the expression of TMEM106 B m RNA was found in malignant meningioma cells,the expression of TMEM106 B in the silent group was decreased,while that in the overexpression group was increased.Western blot was performed for the verification of the expression of protein in different groups of both cell lines: the expression of TMEM106 B in the silent group was decreased,and that in the overexpression group increased,which was up to the requirements of grouping as expected.2.CCK8 and MTT assays showed that the cell proliferation capacity of cell lines in the silent group was decreased,while that was significantly increased in the overexpression group(P<0.01).Flow cytometry: the cell count of G0/G1 phase was decreased and that of S phase was increased in the TMEM106 B overexpression group,and the phenomenon is the opposite in silent group;the cell apoptosis of the silence group increased,while the overexpression group decreased.3.The result of scratch assay and transwell assay indicated that the ability of cell migration and invasion was significantly increased in the overexpression group.4.Silent and overexpressed TMEM106 B,the protein expression levels of PI3 K,p-PI3 K,p-AKT also decreased or increased(P<0.05).Conclusions:1.TMEM106 B promotes the proliferation and improves the ability of migration and invasion of malignant meningioma cells.2.TMEM106 B promotes the cell cycle transform from G0/G1 phase to S phase,and inhibits the cell apoptosis.3.TMEM106 B could activate the PI3K/AKT signaling pathway,this may be the way that TMEM106 B affects the abilities of proliferation,migration and invasion in malignant meningioma cells.