Establishment Of An Orthotopic Lung Cancer Model By Percutaneous Lung Puncture Using Lewis Lung Cancer Tissue Block

Purpose:Despite the cell suspension injection and surgical orthotopic implantation have been widely used to establish orthotopic models of lung cancer,they are flawed in several aspects.The purpose of this research was to validate the feasibility of the modified method--developing an orthotopic model of lung cancer by transthoracic lung puncture with tumor fragments,which we proposed based on the previous studies.Method:According to the method we proposed,a tumor specimen from a subcutaneous model was cut into 1*1*1 mm fragments and engrafted into the left lung lobe of 50BALB/C mice.All the mice were observed for two months on a daily basis after implantation and humanely sacrificed by CO2 inhalation when became moribund from respiratory failure caused by severe tumor burden,prostration and severe lung infections.All the gross specimens were photographed and got the most representative neoplastic lesion for histopathological examination.In order to better monitor the tumor formation in vivo,we selected five mice randomly to accept small animal PET/CT scan.Result:In these models,we could observe local tumors formation,ipsilateral thoracic tissues infiltration,then contralateral chest wall and right lung metastases,and finally distant kidney metastases.The overall rates of tumor growth and metastasis were60.86%(28/46)and 57.14%(16/28),respectively.And three mice accepted small-animal PET/CT scan had local tumor development,but no distant metastasis was observed.Conclusion:This modified method we proposed was reliable,reproducible,minimally invasive,simple and easy to grasp,which could provide the preliminary basis for the establishment of patient-derived orthotopic xenografts of lung cancer.