MVP Plays A Protective Role In Periodontitis By Inhibiting Osteoclast Differentiation And Reducing Bone Resorption

Objective:To investigate the role of major vault protein（MVP）in bone resorption by establishing an experimental periodontitis model.Methods:A model of experimental periodontitis was established by local injection of lipopolysaccharide（LPS）to wild-type mice（WT）and MVP knockout(MVP^-/-)mice.The severity of periodontitis was detected by Micro-CT and then three-dimensional reconstruction and bone morphology analysis were performed.The number of osteoclasts in alveolar bone were performed using tartrate-resistant acid phosphatase（TRAP）staining.In vitro,the role of MVP in osteoclast differentiation was verified by Western Blotting,TRAP staining and Wheat Germ Agglutinin（WGA）staining after cultured with RANKL and M-CSF.All the data were analyzed by Graph Pad Prism 6.0 software.Results:In experimental periodontitis induced by LPS,MVP^-/-group produced more serious periodontitis:the more bone resorption and more osteoclasts could be seen in the injection area.In vitro,experiments showed that MVP was highly expressed during osteoclast differentiation and peak expression was on the 5th day.Furthermore,MVP deletion promoted the number and volume of osteoclasts,as well as bone resorption function.Conclusion:MVP hinders periodontitis by inhibiting osteoclast differentiation and reducing bone resorption.This study provides new ideas for periodontal diseases that may be faced in orthodontic clinical treatment.