The Bystander Effects Of The Neuroglioma Tumors Induced By The Gamma Ray Irradiation

Radiation induced bystander effect,one of radiobiological effect,which widely concerned by people in recent years,makes big contribution to the improvement and development of radiation biology.The research of radiation induced bystander effect and related molecular mechanisms can laid theoretical foundation for the extensive application of radiation protection and nuclear physics,and show important guidance and application foundation in radiation protection,radiation risk assessment and clinical application of radiotherapy.The issue of glioma cells by gamma ray radiation induced bystander effect makes high level of study,hoping to provide a more complete theoretical basis for glioma clinical gamma ray radiotherapy treatment and laid the theoretical foundation a scientific,efficient,reasonable gamma ray radiotherapy program.The issue used gamma ray as ion source ray,and constructed a gamma ray irradiation damage cell model in two kinds of glioma cells U87 MG cells and U251 cells.Medium transfer experiment was used in this issue to research the biological effects and potential molecular mechanisms induced by gamma irradiation at different doses.The results of experiment showed that radiation induced bystander effect can promote the proliferative effect of U87 MG cells in unirradiated glioma cells;however,glioma U251 without gamma irradiation had no proliferative effect.In order to explore the reasons of the proliferation of glioma cells induced by gamma radiation side effects,we also tested to promote proliferation in cell cycle,apoptosis and gene regulation.The results of experiment showed the proliferation was not related to the cell cycle,and it was not achieved by shortening the cell cycle.Results of apoptosis showed that only U87 MG cells display a significant decrease in the early time(<24h).But as time went by(such as 48h),the activity of apoptosis went decreased,with the activity of proliferation going increased significantly.Indicating that apoptosis played an important role in radiation induced side effects in the early time,whereas proliferation of U87 MG cells took a dominant role in radiation induced side effects at 48 h.Therefore,we tried to find the reason why proliferation was promoted in glioma U87 MG cells caused by radiation induced side effects by gamma ray from the perspective of proliferation-related signaling pathways.The results indicated that proliferation promotion in U87 MG cells caused by radiation induced side effects by gamma ray may be achieved via PI3K-AKT signaling pathways.By addition of PI3 K inhibitors can reduce proliferation significantly.And results of immunofluorescence showed that the proliferation reduction was achieved by inhibiting the production of phosphorylated AKT and preventing it from entering the nucleus.At the same time,AKT signaling pathway was also mentioned to the proliferation promotion by exosome and its proteomic analysis.In summary,results of experiment showed that proliferation was different in two kinds of glioma cells and phosphorylated AKT was key to radiation induced side effects by gamma ray.Phosphorylated AKT will be our target to research mechanism of action and molecular in radiation induced side effects.What we researched will provide a theoretical basis to the clinical research of indirect damage effect in radiation biology.