| ObjectivesThe pathogenesis of susceptibility to mental disorders in adulthood due to poor early experiences is not yet fully understood.HPA axis dysfunction,decreased neuroplasticity,and epigenetic changes are the main research hotspots.In recent years,neuroelectrophysiological research has played an important role in the pathogenesis of mental disorders.The mother-infant separation animal model is an important tool for the study of the pathophysiological mechanism of the disease susceptible to depression due to early adverse experiences.Previous studies have shown that Wenyang Jieyu Decoction has a good regulatory effect on behavioral abnormalities,HPA hyperfunction,neurotransmitter changes and decreased neuroplasticity caused by mother-infant-separated mice.Local field potential is a low-frequency(less than 500 Hz)extracellular electrophysiological signal,and it is also a cortical oscillation synchronized by a certain number of neurons in the brain.It explains the process of neural activity on a fine time and space scale and reflects local synapses.The excitability and inhibition of the posterior potential.Therefore,this study took the mother-infant-separated mice as the research object,and explored the effect of hippocampal or amygdala electrical stimulation on the field potential of the mouse’s related brain areas and the regulating effect of Wenyang Jieyu Recipe,which can provide ideas for the prevention and treatment of diseases related to early adverse experiences.Method1.Mark the mouse as PDO on the day of birth,and perform mother-to-child separation(MS)from PD5 to PD 14,and the separation time is 8 hours per day;the mother-to-child separation and the pups are weaned at PD21 and randomly divided into mother-to-child separation groups(MS),Wenyang group(Wenyang),Wenyang Jieyu group(XSF),Jieyu group(Xiaoyao)and Fluoxetine group(FLU),were fed the corresponding medicine mixed feed to adult(PD90),normal The mice in the group were separated every day for 10 minutes,and the mice in the normal group and the mother-infant separation group were fed with developmental feed.2.After the experimental mouse reaches adulthood,conduct social interaction cognitive behavior test and evaluate its behavioral data to determine its behavior pattern.3.The effect of hippocampal electrical stimulation on the field potentials of the relevant brain regions of the mother-infant-separated mice and the effect of Wenyang Jieyu Recipe.4.The effect of electric stimulation of the amygdala on the field potentials of the relevant brain regions of the mother-infant-separated mice and the effect of Wenyang Jieyu Recipe.ResultsChanges of social interaction behavior in mother-infant-separated mice and the regulating effect of Wenyang Jieyu RecipeIn the mouse social interaction cognitive behavior experiment,in terms of training exploration:Compared with NC mice,the training exploration time of MS mice was significantly improved(P<0.01);Compared with the MS group,Xiaoyao,XSF,FLU training exploration The time of the mice was significantly decreased(P<0.05),and Wenyang could significantly down-regulate the training and exploration time of the mother-infant separation mice(P<0.01).In terms of identification and exploration:MS compared with NC group,the identification and exploration time was longer(P<0.01);Compared with MS group,Wenyang,XSF,FLU can significantly down-regulate the time that the mother-infant separated mice stay in the interaction zone in the identification and exploration experiment(P<0.01),and the time of Xiaoyao’s discriminative exploration also decreased significantly(P<0.05).In terms of discrimination rate and far corner exploration:there is no difference in statistics.The effect of hippocampal electrical stimulation on the field potentials of the related brain regions of the mother-infant-separated mice and the regulating effect of Wenyang Jieyu Decoction1.When the hippocampus is electrically stimulated,the power spectral density of the MS group is significantly higher than that of the normal group in the 0-4Hz frequency band of the amygdala,and the difference is statistically significant(P<0.01);Wenyang,XSF,FLU administration group Compared with the MS group,there was a very significant decrease,and the difference was statistically significant(P<0.01);the Xiaoyao group was significantly lower than the MS group,and the difference was statistically significant(P<0.05).In the 12-30Hz frequency band of the amygdala,the power spectral density of the MS group was significantly higher than that of the normal group,and the difference was statistically significant(P<0.05);the Wenyang and XSF groups were significantly lower than the MS group,and the difference was statistically significant(P<0.05).When the hippocampus was stimulated,the amygdala was seen.The field potential energy density of the MS group was significantly higher than that of the NC group at 5s,and the color was brighter(P<0.01);Xiaoyao and FLU were significantly lower than the MS energy density(P<0.05);XSF Compared with the MS group,the energy density was extremely significantly lower,and the difference was statistically significant(P<0.01).2.When the hippocampus was stimulated,the power spectral density of the prefrontal cortex in the MS group at 4～8Hz increased significantly compared with the normal group,and the difference was statistically significant(P<0.01);the Wenyang and FLU administration groups decreased significantly compared with the MS group,The difference was statistically significant(P<0.05);the XSF group was significantly lower than the MS group,and the difference was statistically significant(P<0.01).The power spectral density of the prefrontal cortex in the MS group at 50～125Hz was significantly higher than that in the normal group,and the difference was statistically significant(P<0.01);the Wenyang,XSF,and FLU administration groups were significantly lower than the MS group,and the difference was statistically significant(P<0.05).When the hippocampus was stimulated,the prefrontal cortex area of mice was seen.The field potential energy density of the MS group was significantly lower than that of the NC group at 5s,and the difference was statistically significant(P<0.01);the energy density of the Xiaoyao and FLU groups was significantly higher than that of the MS group(P<0.05);The XSF group was significantly higher than the MS group,and the difference was statistically significant(P<0.01).When the hippocampus was stimulated,the prefrontal cortex theta-low gamma phase amplitude coupling was significantly enhanced in the MS group compared with the NC group,and the difference was statistically significant(P<0.01);XSF and FLU were significantly weaker than the MS group in oscillation,and the difference was statistically significant(P<0.05).3.When the hippocampus was stimulated,the average phase consistency between the amygdala and the prefrontal cortex at 4～8Hz,the MS group had a slight increase compared with the NC group,the difference was not statistically significant(P>0.05);Wenyang,Xiaoyao,XSF,FLU There was no significant change in the average phase consistency value of the MS group compared with the MS group.When the hippocampus was stimulated,the correlation coefficient between the amygdala and the prefrontal cortex at 4-8Hz,MS was significantly higher than that of NC,and the difference was statistically significant(P<0.05);Wenyang,Xiaoyao,XSF,and FLU had correlation coefficients compared with MS group Increased,but the difference in the other groups except the XSF group was not statistically significant.Effect of electrical stimulation of amygdala on field potentials in related brain regions of mother-infant-separated mice and the regulating effect of Wenyang Jieyu Decoction1.When the amygdala is stimulated,the prefrontal cortex is in the 0～4Hz frequency band,MS has a significant decrease compared with the normal group,and the difference is statistically significant(P<0.05);the Xiaoyao administration group has a significant increase compared with the MS group,and the difference is statistically significant(P<0.05);The Wenyang,XSF,and FLU groups showed an upward trend compared with the MS group,and the difference was not statistically significant.When the amygdala was stimulated,the mouse prefrontal cortex area was seen.The field potential energy density of MS was significantly lower than that of NC in 5s,and the color was darker(P<0.01);the energy density of XSF administration group was significantly higher than that of MS,and the difference was statistically significant.Significance(P<0.05).When the amygdala was stimulated,the theta-gamma phase amplitude coupling of the prefrontal cortex was significantly lower in the MS group than in the NC group,and the difference was statistically significant(P<0.05).2.When the amygdala is stimulated,the hippocampus is in the 4～8Hz frequency band,MS has a significant decrease compared with the normal group,and the difference is statistically significant(P<0.05);the XSF administration group has a significant increase compared with the MS group,and the difference is statistically significant(P<0.05);The Wenyang,Xiaoyao,and FLU groups showed an upward trend compared with the MS group,and the difference was not statistically significant(P>0.05).When the amygdala was stimulated,the field potential energy density of 5s in the hippocampus of mice was significantly lower than that of NC,and the color was darker(P<0.01);the energy density of XSF was significantly higher than that of MS group,and the difference was statistically significant(P<0.05)).3.When the amygdala was stimulated,the average phase consistency value of hippocampus and prefrontal cortex at 4～8Hz,the MS group was significantly lower than the NC group,the difference was statistically significant(P<0.01);Wenyang,Xiaoyao,XSF,FLU Compared with the MS group,the average phase consistency value of the MS group increased,but the Wenyang difference was not statistically significant,and the differences in the other groups were statistically significant.When the amygdala was stimulated,the correlation coefficient between hippocampus and prefrontal cortex at 4～8Hz,MS was significantly lower than that of NC,and the difference was statistically significant(P<0.05);XSF was significantly higher than that of MS,and the difference was statistically significant(P<0.05);The correlation coefficients of Wenyang,Xiaoyao,and FLU all increased compared with the MS group,and the difference was not statistically significant(P>0.05).ConclusionMice with mother-infant separation showed a decline in social cognitive function;and mother-infant separation led to a decrease in the field potential energy density of the mouse medial prefrontal cortex and hippocampus,and an increase in the field potential energy density of the amygdala,which also resulted in the prefrontal cortex,hippocampus,and amygdala.The synchrony of theta rhythms in the three brain regions is reduced.Wenyang Jieyu Recipe not only improves the abnormal discharge of the hippocampus-prefrontal cortex-amygdala neural circuit in mother-infant separated mice;it also improves the decline of social cognitive function in mother-infant separated mice. |
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