The Experimental Study:Q-switched Nd:YAG 1064nm Laser Combined With Topical Metformin On The Melasma Model Of Mouse

Objective:To discuss the effectiveness and safety of Q-switched Nd:YAG 1064nm laser combined with topical 30%metformin lotion in the treatment of the melasma model of mouse,and to initially explore the possible mechanism of topical metformin in the treatment of melasma,in order to provide relevant experimental evidence for clinical treatment of melasma and pigmented dermatitis.Methods:In this study,the melasma model of mouseis established by irradiation of UVB and intramuscular injection of progesterone.The mice in the model group are randomly divided into 4 groups,namely the control group(Control),the laser group(Laser),and the metformin group(Met),laser combined with metformin group(Laser+Met),the course of treatment in each group is 4 weeks..Detect the number of melanocytes and the activity of melanin and tyrosinase by immunohistochemistry and western blot.Results:After 4 weeks of treatment,the immunohistochemical results show that compared with the control group,the number of melanocytes,melanin and tyrosinase expression of mice in the laser group,metformin group,and combination group are significantly reduced,the difference is statistically significant(P<0.001);among them,the difference is not statistically significant between the laser group and the metformin group(P>0.05),and the difference is statistically significant between the combination group and the laser group,the metformin group(P<0.001).Western blotting results show that compared with the control group,the expression of melanin and tyrosinase protein in the laser group,metformin group,and combination group are significantly reduced,the difference is statistically significant(P<0.001);among them,the difference is not statistically significant between the laser group and metformin group(p>0.05),and the difference is statistically significant between the combination group and the laser group,the metformin group(P<0.001).Conclusion:Q-switched Nd:YAG 1064nm laser combined with topical 30%metformin lotion have a significant effect of the melasma model of mouse,it could shorten the course of treatment and has higher safety,and the combined application is better than its single application.The mechanism of topical metformin in the treatment of the melasma model of mouse may be related to the reduction of melanin and tyrosinase synthesis in the skin.