The Mechanism Of MEHP Affects The Migration Of Human Prepuce Fibroblasts

Objective:1.To clarify the effect and regulatory mechanism of MEHP on proliferation,apoptosis and migration of HFF-1 cells.2.To explore the mechanism of JNK in MEHP regulating the migration of HFF-1 cells.Method:1.To observe the proliferation,apoptosis and migration changes of HFF-1 cells in each group by setting up control group and different concentration MEHP intervention group interposed in 24 h,48h.2.To examine the effects of MEHP on the transcription and expression of JNK in HFF-1 cells.3.We add a specific JNK inhibitor(SP600125),to detect the effect of MEHP and SP600125 on the activation of JNK protein in HFF-1 cells by Wb.4.To investigate the effect of activation of JNK protein in the migration of HFF-1 cells by scratch test.Result:1.The apoptosis of HFF-1 cell was significantly promoted after 24h/48 h MEHP exposure at different concentrations,but had no obvious effect on cell proliferation.2.The migration of HFF-1 cell was significantly inhibited after MEHP exposure,and the inhibitory effect was positively correlated with MEHP exposure dose.3.The levels of JNK gene transcription and protein translation were significantly up regulated in HFF-1 cells when exposed by MEHP.4.Phosphorylated JNK1/2 protein activation in HFF-1 cells was significantly promoted after MEHP exposure.5.HFF-1 cell migration was further inhibited when phosphorylated JNK was blocked and MEHP was exposed.Conclusion:1.Migration of prepuce fibroblasts was inhibited when MEHP was exposed,which may be an important reason to prevent normal tubular fusion of prepuce.2.Migration of prepuce fibroblasts was inhibited when JNK phosphorylation was abnormal,up-regulated or down-regulated,which may be an important mechanism regulating hypospadias.