CK13 Gene In Nasopharyngeal Carcinoma Cell HNE1 Radiation Sensitivity Influence Mechanism Research

Objective:Further to understand the mechanism of NPC radiation sensitivity,explore new targets in order to effectively increase NPC radiation sensitivity,thus improve the NPC radiotherapy curative effect reduce local residual and metastasis,reduce complications,has important clinical significance,this study to clarify and reveal CK13 regulation of cell cycle related signaling pathways that affect how the radiation sensitivity of nasopharyngeal carcinoma’s HNE1,further guide clinical targeted therapy,in order to reduce the radiation resistance,improve the patients survival rate.Methods:HNE1 cell can be divided into the control group,the anti-CK13a group and anti-CK13b(CK13 Knockdown)and control group rapamycin treatment group(100 nmol/L rapamycin processing 1 h),anti-CK13 a+rapamycin treatment group(100 nmol/L rapamycin processing 1 h),were treated with radiation therapy(200 cGy/5 min agent amount of exposure),CCK 8 method is used to inspect each cell proliferation ability,with Annexin V/PI staining flow cytometry to detect each cell apoptosis rate,qPCR method to detect PI3K/AKT/mTOR signaling pathways related to the expression of PTEN gene,Western blotting method to detect PI3K/AKT/mTOR signaling pathway related protein expression.Results:1.CCK 8,according to the results of nasopharyngeal carcinoma(NPC)after radiation treatment,knock on low CK13 HNE1 cell growth was significantly increased(P<0.01),Western blotting,according to the results of type low cell proliferation protein expression of CyclinD1 CK13 group(a:2.63±0.44;b:2.62±0.32)than the control group(1.0±0.19)significantly increased(P<0.05).2.Compared with the control group(1.0±0.04),low CK13 group(a:0.69±0.02;b:0.52±0.03)can effectively reduce the iconic HNE1 cell radiation under the conditions of nasopharyngeal carcinoma apoptosis protein Cleavedcaspase-3 protein expression(a:P<0.05;b:P<0.01);In addition,compared with the control group(1.0±0.05),Knock-down CK13 group(a:0.56±0.06;b:0.43±0.02)can effectively reduce the nasopharyngeal carcinoma HNE1 cell markers gamma radiation under the conditions of DNA damage,the γ H2AX protein expression(P<0.01)3.Western blotting showed that compared with the control group(1.0±0.14),Knock-down CK13 group(a:2.65±0.02;b:2.92±0.32)can effectively increase HNE1 nasopharyngeal carcinoma(NPC)cells radiation under the conditions of p-AKT/AKT protein expression(all p<0.01);Compared with the control group(1.0±0.16)at the same time,on low CK13 group(a:2.71±0.26;b:2.62±0.21)can effectively increase HNE1 nasopharyngeal carcinoma cells radiation under the conditions of p-S6K/S6K protein expression(p<0.01).Further testing found that join PI3K/AKT/mTOR signaling pathway inhibitor rapamycin can save cell proliferation due to low CK13 knock phenomenon of enhanced(P<0.05)Conclusions:1.Knockdown CK13 HNE1 cell lines of nasopharyngeal carcinoma(NPC)can promote proliferation and reduce HNE1 cell lines of nasopharyngeal carcinoma radiotherapy sensitivity.2.The results show that the Knockdown CK13 can reduce the apoptosis rate of radiotherapy for nasopharyngeal carcinoma cells HNE1 and DNA damage.3.Reduce CK13 likely by enhancing PI3K/AKT/mTOR signaling pathway activity to improve the radiation resistance of nasopharyngeal carcinoma cells...