| Objective:A block randomized,parallel-controlled research methods was conducted to investigate the efficacy of Kuijie enema Ⅱ regulating antimicrobial peptides inhibiting inflammatory response in the treatment of ulcerative colitis.Research methods:According to the diagnostic criteria and inclusion criteria,100 patients with mild to moderate ulcerative colitis of damp-heat type of large intestine were collected from outpatient and ward of Nanjing Hostital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine from October 2019 to October 2020.A total of 100 cases were finally included and randomly divided into the test group(50 cases)and the control group(50cases).The test group was given Kuijie enema Ⅱ combined with oral mesalazine sustained-release granules,and the control group was given mesalazine suppository combined with oral mesalazine sustained-release granules.The course of treatment was 8 weeks.Finally,the common data of patients,curative effect evaluation index(clinical effective rate,clinical remission rate,modified Mayo score,TCM syndrome curative effect),inflammatory factors(WBC、NEUT、CRP、ESR、IL-6、TNF-α),antibacterial peptide related indicators(CAMP/LL-37、DEF-a1、HBD-2、HBD-3)fecal calprotectin,nutritional status indicators(Hb,ALB)were observed and analyzed.Results:1.Curative effect analysis:(1)clinical effective rate:After treatment,the clinical effective rate of the test group was 86.67%,and the clinical effective rate of the control group was 63.33%.The comparison between the two groups was statistically significant(P<0.05);(2)clinical remission rate:After treatment,the clinical remission rate of the test group was 56.67%,and the clinical remission rate of the control group was 30%.The test group was better than the control group,and the comparison between the two groups was statistically significant(P<0.05);(3)modified Mayo score:After treatment,the score of the test group was statistically significant compared with the score of the control group(P<0.05),and the decline of the test group was better than that of the control group;(4)TCM syndrome curative effect:After treatment,in the experimental group,16 cases were markedly effective,31 cases were effective,3 cases were ineffective,and the total effective rate was 94%.In the control group,6 cases were markedly effective,32 cases were effective,12 cases were ineffective,and the total effective rate was 76%.The difference between the two groups was statistically significant(P<0.05).In conclusion,when the basic medication is the same,Kuijie enema II is superior to western medicine alone in clinical effiective rate,clinical symptoms improvement,endoscopic remission and TCM symptoms improvement.2.Laboratory physical and chemical results:(1)inflammatory factor analysis:Before treatment,the test group of WBC,NEUT,CRP,ESR,IL-6,TNF-α was not statistically significant compared with the control group(P>0.05),and it was comparable.After treatment,the test group was better than the control group.(P<0.05),indicating that the test group is better than the control group in improving inflammatory factors;(2)antibacterial peptide related indicators:Before treatment,the CAMP/LL-37,DEF-al,HBD-2,and HBD-3 in test group were not statistically significant compared with the control group(P>0.05)and were comparable.After treatment,the test group had a better decline The control group(P<0.05),indicating that the test group is better than the control group in improving antimicrobial peptides;(3)fecal calprotectin:Before treatment,the test group was compared with the control group,P>0.05,which was not statistically significant and comparable;after treatment,the comparison between the two groups was P<0.05,which was statistically significant,indicating that the test group and the control group after treatment Fecal calprotectin levels were lower than before;after treatment,the test group compared with the control group,P>0.05,no statistical significance;(4)nutritional status indicators:After the treatment of Hb and ALB,the comparison between the two groups was P<0.05,which was statistically significant,indicating that the Hb and ALB of the two groups showed an upward trend after treatment,and the nutritional status was improved;after treatment,the experimental group and the control group were similar Ratio,P>0.05,did not become statistically significant after treatment.3.Safety analysis:Before and after treatment,the patient had no serious adverse reactions,and the liver and kidney function and electrocardiogram were not abnormal.Research conclusion:(1)Under the same basic medication,Kuijie enema Ⅱ is effective in treating mild to moderate colorectal damp-heat ulcerative colitis.It is superior to the simple western medicine group in terms of clinical symptom relief,improvement of TCM syndromes,reduction of antimicrobial peptides and inflammatory factors,and is worthy of clinical promotion;(2)After treatment,the levels of antimicrobial peptides and inflammatory factors decreased synchronously than before,suggesting that Kuijie enema II can reduce inflammation by regulating the levels of antimicrobial peptides to treat ulcerative colitis.The mechanism needs to be further verified through basic experiments. |
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