Decoding The Core Components And Uncovering The Molecular Mechanism Of LXD In Treating Uveitis

Background and PurposeLongdan Xiegan Decoction(LXD)is a famous herbal formula in China,which has been widely used to treat inflammatory diseases for centuries.It has been proved that LXD has obvious inhibitory effect on inflammatory cells associated with Uveitis.However,the core functional component group and its possible mechanism are not clear at present.To optimize LXD,we try to find the core functional components and speculate the possible mechanism.MethodsInitially,the functional communities related to the pathogenic genes of Uveitis are obtained and integrated together by using the community model of the network.Secondly,an optimization space is made by using the importance of nodes,which was used to find the key functional components together with the reverse prediction model.Finally,we analyze the mechanism of key functional groups.ResultsIn the C-T-P-D(Component-Target-PPI(Protein-Protein Interaction)-disease)network,we found eight KFCs with important functional significance by using unique algorithms and models.After verification,we know that KFCs can represent the corresponding C-T network for treating Uveitis.A total of 195 active components and 56 target components were screened.In addition,the enrichment results showed that 349 effective proteins and the signaling pathways of IL-17,Toll-like receptor and T cell receptor played an important role in the pathogenesis of Uveitis.This research explored the multi-gene pharmacological mechanism of action of LXD against Uveitis through network pharmacology.The findings provide new ideas for research on the mechanism of action of Chinese medicine against Uveitis.Conclusion:To sum up,a method based on integrated system pharmacology is constructed to capture core components and decipher the mechanism of LXD on Uveitis.In addition,our proposed Key Functional Communities(KFCs)prediction and verification strategy provides a methodological reference for optimizing the core component group and explaining the molecular mechanism of Traditional Chinese Medicine(TCM)in treating of complex diseases.