| Purpose: This study intends to use electrospinning technology to combine gelatin(GT)and polycaprolactone(PCL)to prepare nanofiber membranes,and use 3D printing technology to construct polylactic acid(PLA)scaffolds.Combine the two with the second-generation chondrocytes to prepare a cartilage composite scaffold,and analyze the feasibility of this type of scaffold for repairing cartilage damage through animal experiments,providing an important basis for the use of tissue engineering methods to treat cartilage tissue damage diseases.Methods: First,the chondrocytes are cultured and passaged;the GT and PCL mixed solution is made into nanofiber membranes using electrospinning technology;the PLA scaffold is prepared according to the model parameters using a 3D printer.Secondly,the second-generation chondrocytes were dropped onto the GT/PCL electrospun nanofiber membrane,and the tissue engineered cartilage membrane scaffold was constructed by the superposition method,and implanted in nude mice for culture,and was isolated at the 3rd and 6th weeks.Appearance morphology observation and histological staining identification.Finally,the GT/PCL membrane loaded with cartilage cells was combined with the PLA scaffold to prepare a tissue engineering cartilage scaffold complex,which was implanted in nude mice for in vivo culture,and was isolated at the3 rd,6th,and 9th week,and the appearance and morphology were observed.And histological staining identification.Results: After the perichondrial membrane scaffold group was cultured in nude mice,the appearance morphology observation and histological staining identification showed that there were abundant chondrocytes and cartilage matrix formation;the cartilage scaffold complex group was implanted in nude mice and cultured in vitro.The histological staining identification showed more abundant chondrocytes and matrix formation than the perichondrial sheet scaffold group,and it was better in appearance and mechanical strength.The native cartilage tissue is simulated.Conclusion: It is feasible to construct tissue engineering cartilage scaffolds using chondrocyte-GT/PCL electrospun nanomembrane composites combined with 3D printed polylactic acid scaffolds,which provides theoretical support for the use of tissue engineering methods to repair cartilage tissue damage diseases. |
