| Objective: To detect the concentration of NMU(Neuromedin U)in peripheral blood and explore the clinical significance of NMU in lung cancer.Methods: A total of 160 cases in the Second Hospital of Dalian Medical University from July to December 2020 were collected,including 110 lung cancer patients 、 4 benign pulmonary nodules patients who were hospitalized and 44 healthy people who had a physical examination.The concentration of NMU in serum was detected by competitive inhibition ELISA(enzyme-linked immunosorbent assay)after extracting the peripheral blood of the subjects.In this study,the subjects were divided into three groups: the early lung cancer group(including TNM I and II),the advanced lung cancer group(including TNM III and IV)and the control group(including patients with benign pulmonary nodules and healthy subjects)to explore the difference of NMU concentration among them.In the advanced lung cancer group,the relationship between the concentration of NMU and the general data,the clinicopathological data,different organ metastasis of lung cancer was studied.And we explored the relationship between the concentration of NMU and the T stage,N stage,M stage,the number of lymph node metastasis,the number of organ metastasis,the history of operation,the history of chemotherapy.And evaluated whether the NMU level is related to the expressions of 6 traditonal tumor markers detected when the lung cancer patiences were hospitalized or not,such as SCC(squamous cell carcinoma antigen),CEA(carcinoembryonic antigen),NSE(neuron-specific enolase),CYFRA21-1(cytokeratin fragment 19),CA199(carbohydrate antigen 199)and CA125(carbohydrate antigen 125).Results: 1.There was a significant difference in the levels of NMU among the three groups(χ~2=32.511,P=0.000 *).Pairwise comparison showed that:(1)the concentration of NMU in the advanced lung cancer group(814 ±340pg / ml)was significantly higher than that of the early lung cancer group and the control group(P = 0.000 *,P = 0.000 *),and the difference was statistically significant;(2)there was no significant difference in NMU levels between the early lung cancer group(593 ±264pg / ml)and the control group(571 ±231pg/ml)(P = 0.525).2.Compared NMU levels among four TNM stages,it was found that there was a significant difference in NMU levels among the four stage(P= 0.000*): The NMU levels of TNM stage III(705 ± 295 pg / ml)and IV(858 ± 350 pg / ml)were higher than that in TNM stage I(592 ± 274 pg / ml),the difference was statistically significant(P = 0.027 *;0.000 *).3.Compared NMU levels of the T,N and M stage showed that:(1)there was a significant difference in NMU levels among four N stages(F = 4.299,P = 0.007 *).Pairwise comparison found that the NMU levels in stage N2(753 ±168pg / ml)and N3(799 ±268pg / ml)were higher than that of N0(672 ±358pg / ml).The difference was statistically significant(P = 0.013 *;0.002 *);(2)there was a significant difference in the NMU levels of M stages(t = 4.748,P = 0.001).The level of NMU in stage M1(833 ±310pg / ml)was significantly higher than that in M0(627 ±276pg / ml),the difference was statistically significant(P = 0.000 *);(3)there was no significant difference in the concentration of NMU among T sages.4.Analyzed the relationship between the level of NMU and the number of lymph node metastasis、organ metastasis: the level of NMU was significantly correlated with the number of lymph node metastasis and the number of organ metastasis(the correlation coefficient was 0.265 and 0.432,P<0.05*),and the NMU level rised with the increase of the number of lymph node and organ metastasis.5.The relationship between the NMU level and different metastatic organs was analyzed in advanced lung cancer group: it was found that the level of NMU is related to the pericardial metastasis,and the NMU level of patients with pericardial metastasis was higher than that of patients without it(z = 2.076,P=0.038*).But there was no significant difference in the NMU level between patients with or without lung metastasis,brain metastasis,liver metastasis,bone metastasis,macrovascular metastasis,renal metastasis and malignant pleural effusion metastasis.6.The relationship between the NMU level and different tumor markers was analyzed in lung cancer group: it was found that the level of NMU was significant ly correlated with the levels of CA125(the correlation coefficient was 0.474,P = 0.000 *),CYFRA21-1(the correlation coefficient was 0.333,P = 0.000 *)and CEA(the correlation coefficient was 0.263,P = 0.005 *),but not with the levels of CA199,NSE and SCC.7.In the advanced lung cancer group,there was no significant difference in the NMU levels among sex,age,smoking history,drinking history,tumor family history,location,pathological type and degree of differentiation(P > 0.05).8.In advanced lung cancer group,there was no significant difference in the NMU levels among operation,chemotherapy and chemotherapy time(P > 0.05).Conclusion: 1.The level of NMU in patients with advanced lung cancer was higher than that in patients with early lung cancer and control group,indicating that NMU may be used as a tumor marker for the diagnosis of advanced lung cancer and associated with poor prognosis.2.The levels of NMU in stage N2 and N3 were higher than that in stage N0,and the level of NMU in stage M1 was higher than that in stage M0.At the same time,the level of NMU rised with the increase of the number of lymph node metastasis and organ metastasis,indicating that the monitoring of NMU concentration in patients with lung cancer can predict the occurrence of lymph node metastasis、distant metastasis and the progress of the disease. |
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