| Mast cells have been shown to play an important role in the defensive immune response and immune regulation,and can also regulate the pathological process of a variety of allergic diseases.Some studies have shown that the presence of MC in tumor tissue promotes the occurrence and development of tumors,but some studies have put forward the opposite view,High mast cell density in tumor tissue is associated with longer survival.The role of mast cells in the immune response of colorectal tumors is still not clear.The definition of circulating MCP marker also provides a new research direction.Objective:In this study,immunology,cytology and other experimental techniques were used to clarify the expression and distribution characteristics of MC in CRC patients,analyze the correlation between MC and clinical indicators of CRC patients,and explore the relationship between MC expression level in CRC tissues and prognosis.Methods:1.A total of 33 patients with newly diagnosed colorectal cancer were enrolled in this study.All patients did not receive neoadjuvant chemoradiotherapy before surgery,and patients with infectious diseases,autoimmune diseases,or multiple primary cancers were excluded.Blood samples were taken before surgery.Fresh bowel tumor and nontumor tissues(at least 5cm from the tumor site)were collected and isolated from 14 patients after radical colorectal cancer resection.Blood samples were collected from 13 patients on the 7th day after surgery.In addition,10 gender-matched healthy controls were recruited in this study,and their blood samples were taken as healthy control blood samples,which were matched with the gender and age of the above-mentioned colorectal cancer patients.Four gender-matched patients with mixed hemorrhoids and rectal protrusion were recruited and their tissue samples were taken after surgery.2.Expression of MC and its progenitor cells in peripheral blood mononuclear cells(PBMC),tumor center tissues and non-tumor tissues of CRC patients were detected by flow cytometry.PBMC was extracted from healthy controls(HC)as control.3.TNM staging system for colorectal tumors and serum carcinoembryonic antigen(CEA)indexes were introduced to facilitate the analysis of the correlation between MC and clinical indicators of CRC patients.Results:1.Compared with early colorectal cancer patients,the frequency of mast cell progenitor cells in peripheral blood circulation was lower in advanced patients,while the number of mast cells in the tumor was higher.It is suggested that mast cell progenitor cells may infiltrate from blood to colorectal tumor.2.The number of MC in tumor tissues of CRC patients was significantly lower than that of non-tumor tissues,and the frequency of MC in tumor tissues of advanced CRC patients was significantly higher than that of early CRC patients.Meanwhile,we found that the frequency of MC in CRC tumor tissues was positively correlated with the level of CEA.3.Circulating MCP was negatively correlated with tumor MC,but not with nontumor MC.Conclusions:1.It was found for the first time that compared with early colorectal cancer patients,the frequency of mast cell progenitor cells in peripheral blood circulation in advanced patients was lower,while mast cells in the tumor were higher.It is suggested that mast cell progenitor cells may infiltrate from blood to colorectal tumor.2.The number of mast cell progenitor cells in the peripheral blood of patients with colorectal cancer was negatively correlated with TNM staging,suggesting that mast cell progenitor cells are a potential biomarker for evaluating colorectal cancer staging.3.On the 7th day after surgery,the frequency of circulating mast cell progenitor cells increased significantly,suggesting that the chemokines secreted after colorectal tumor resection decreased significantly.4.The frequency of circulating mast cell progenitor cells was positively correlated with the occurrence of colorectal cancer,and negatively correlated with tumor infiltration of mast cells. |
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