Study On The Relationship Between SPATA48 Gene And 3 Candidate MiRNA Gene Polymorphisms And Spermatogenesis Disorders

Objective: In order to initially understand the relationship between the SPATA 48,mi R-130 a,mi R-26a-1,mi R-27 a gene mutations and human spermatogenesis disorders.Further to provide new reference materials for research on the genetic causes of male infertility and spermatogenesis disorders.This study investigated the correlation between the polymorphisms of the single nucleotide polymorphism site of the above four genes with azoospermia and oligospermia.Methods:In view of the above reasons,this study used Snapshot,polymerase chain reaction(PCR),restriction fragment length polymorphism(RFLP)and DNA sequencing in normal men and patients with spermatogenesis disorders.Methods: The common single nucleotide polymorphisms of SPATA 48 genes(rs12672941,rs998928 and rs1456908),mi R-130a(rs731384),mi R-26a-1(rs7372209)and mi R-27a(rs895819)gene common single nucleotide polymorphism(singlenucleotide polymorphism,SNP)distribution analysis and comparison.The results:The allele C frequency(28.5% vs.22.8%,P = 0.002,OR = 0.632,95% CI0.475-0.842)and TC+CC genotype frequency(49.8% vs.40.6%,P = 0.037,OR = 1.453,95%CI1.023-2.063)of the SNP rs12672941 locus of SPATA 48 gene in patients with oligospermiawas significantly higher than that of normal men,and the TT genotype frequency(50.1% vs.59.4%,P = 0.037,OR = 0.688,95% CI 0.485-0.978)was significantly lower than normal men.The SNP rs1456908 locus of the SPATA 48 gene has the A allele frequency(23.8% vs.17.9%,P = 0.021,OR = 0.699,95% CI 0.515-0.949)in oligospermia patientsand AG+AA genotype frequency(43.3% vs.33.3%,P = 0.020,OR = 1.532,95% CI 1.068-2.196)was significantly higher than that of normal men,GG genotype frequency(56.7% vs.66.7%,P = 0.020,OR = 0.653,95%CI 0.455-0.936)is significantly lower than normal men.The frequency of the SNP rs7372209 of mi R-26a-1 gene in azoospermic patients was significantly higher than that of TT genotype(7.1% vs.2.1%,P = 0.011,OR = 3.523,95% CI 1.258-9.868)normal men;The T allele frequency(25.8% vs.20.3%,P = 0.038,OR = 1.366,95% CI1.017-1.833)of this locus in patients with oligospermia and TT genotype frequency(7.5% vs.2.1 %,P = 0.006,OR = 3.728,95% CI 1.383-10.047)was significantly higher than that of normal men.Conclusion: The above study suggests that the mononucleotide polymorphism of the SPATA 48 gene has a correlation with Oligospermia: The TT genotype at rs12672941 and the GG genotype at rs1456908 may be protective genotypes to reduce the risk of Oligospermia,and The allele C and allele A at these two SNP may increase the risk of male oligospermia.The TT genotype of the mi R-26a-1 gene of the rs7372209 site may increase the risk of Azoospermia and Oligozoospermia,and the T allele may be a susceptibility gene for male Oligospermia.