Effects Of CD98 Gene Silencing On Proliferation,apoptosis And PI3K/AKT Pathway Of Melanoma B16-F10 Cells

ObjectiveTo investigate the effects of CD98 gene silencing on proliferation,apoptosis and PI3K/AKT pathway of melanoma B16-F10 cells.MethodsshRNA CD98 vector was constructed and transfected into Melanoma B16-F10 cells B16-F10 cells.The cells were randomly divided into three groups:Control group,shRNA-NC group and CD98-shRNA3 group for subsequent experiments.Cell proliferation was detected by clone formation assay.Flow cytometry was used to detect apoptosis.Transwell detects cell invasion.Western blot was used to detect the protein expression levels of CD98,Ki67,PCNA,Bax,Bcl-2,Caspase-3,PI3 K,p-PI3 K,AKT,and p-AKT.ResultsResults show that compared with the Control group,group CD98-shRNA3 CD98 protein expression level decreased significantly(P<0.05),clone formation rate decreased significantly(P < 0.05),but Ki67,PCNA protein expression level decreased significantly(P<0.05),the Melanoma apoptosis rate increased significantly(P<0.05),Bax/Bcl-2,cleaved Caspase-3/Caspase-3 ratio significantly higher(P<0.05),invasive cells was significantly reduced(P<0.05),The ratio of p-PI3 K /PI3 K and p-AKT/AKT was significantly reduced(P<0.05).ConclusionCD98 silencing may inhibit the proliferation and invasion of melanoma B16-F10 cells and promote apoptosis by inhibiting the activation of PI3K/ATK signaling pathway.