Study On The Mechanism Of Autophagy Chloroquine Enhancing Catalpol Promoting Apoptosis Of Gastric Cancer Cells

ObjectiveTo study the effect of Catalpol on gastric cancer cells after inhibiting the autophagy of human gastric cancer AGS cells.MethodsThe AGS cells were treated with Catalpol at concentrations of 10,20,40,80,and 160 μmol/L for 24 h.The inhibitory effect of Catalpol on AGS gastric cancer cells proliferation was observed by CCK-8 method;Western blot detection of Catalpol on autophagy-related proteins LC3 B,Beclin-1 and ATG12 protein expression;Western blot to detect the effect of Catalpol combined with the autophagy inhibitor Chloroquine(CQ)on the expression of autophagy-related proteins LC3 B,Beclin-1 and ATG12 protein and apoptosis Effects of related proteins Bax and Bcl-2;Transwell and scratch test to detect the migration ability of gastric cancer cells after combination of autophagy inhibitor chloroquine;Hoechst 33342 staining and flow cytometry analysis of apoptosis of gastric cancer cells after combination with autophagy inhibitor chloroquine Circumstances;the amount of ROS generation was tested for the production of reactive oxygen species in gastric cancer cells after being combined with the autophagy inhibitor chloroquine.ResultsCCK-8 results show that Catalpol can significantly inhibit the proliferation of gastric cancer cells in a dose-dependent manner(P<0.05).Western blot results showed that with the increase of Catalpol concentration,the expression of autophagy-related proteins LC3 B,Beclin-1 and ATG12 protein were dose-dependently up-regulated(P<0.05),suggesting that Catalpol can cause human gastric cancer The occurrence of cell autophagy;the combined use of Catalpol and the autophagy inhibitor chloroquine can reduce the expression of autophagy-related proteins LC3 B,Beclin-1 and ATG12,further verifying the promotion effect of Catalpol on cell autophagy and chloroquine can inhibit catalpa The autophagy ability of alcohol on gastric cancer cells;the expression results of apoptosis-related proteins Bax and Bcl-2 protein showed that inhibition of Catalpol’s autophagy on gastric cancer cells can better inhibit the growth of gastric cancer cells.Transwell and scratch test results show that inhibition of Catalpol as an autophagy for gastric cancer cells can better inhibit the migration ability of gastric cancer cells.Hoechst33342 staining and flow cytometry analysis results showed that inhibition of Catalpol’s autophagy on gastric cancer cells can better promote the apoptosis of gastric cancer cells.The results of ROS production showed that after inhibiting the autophagy effect of Catalpol on gastric cancer cells,the production of ROS increased,thereby promoting the apoptosis of gastric cancer cells.ConclusionsInhibiting the autophagy effect of Catalpol on gastric cancer cells can increase the apoptosis of gastric cancer cells and inhibit the proliferation of gastric cancer cells.