Study On The Correlation Between Automated Detection Of Ureaplasma Urealyticum/Ureaplasma Parvum And Infectious Diseases Of Genital Pathogen

Ureaplasma is one common type of parasite or commensal in female lower genital tract which is one of the important pathogens that cause genitourinary infectious diseases.In addition to urogenital infections in women with Ureaplasma infection,there is also a great possibility of transmitting the pathogen to sexual partners.Ureaplasma infection is also one of the important causes of infertility and infertility.If pregnant women are infected with Ureaplasma,it may also lead to premature rupture of membranes,premature delivery,neonatal respiratory distress syndrome,postpartum infection and other adverse pregnancy consequences.Ureaplasma known to be related to human disease is divided into 2 biological groups and 14 serotypes.Organism group Ⅰ is Ureaplasma urealyticum(Uu),which includes 10 serotypes with larger genomes:2,4,5,7,8,9,10,11,12,and 13.Biological group II is Ureaplasma parvum(Up),including four serotypes 1,3,6 and 14 with smaller genomes.However,the current clinical testing kits cannot differentiate between Uu and Up,and there is also a lack of research on the difference in pathogenicity between the two biological groups.Especially,there are few reports on Up involved within the genital infectionsThis study uses the newly developed Ureaplasma urealyticum/Ureaplasma parvum joint test kit provided by QIAGEN,which is combined with a complete automatic nucleic acid extraction and purification analyzer.Using this kit,we performed clustering/combined detection of these two pathogens in the patients with clinically determined urogenital diseases.In our work,a standardized process for automatic nucleic acid extraction and detection,and real-time fluorescent quantitative PCR analysis was established.Compared with the "gold standard" culture method that has been used,the new method not only shortens the detection cycle,but also effectively avoids contamination and false negatives.After that,a total of 321 patient samples were collected in the experiment,of which 48 were male samples,and the types of specimens were urethral swab;273 were female samples,and the types of specimens were cervical swab.Fifty-five healthy control samples were all female cervical swab samples.In view of the results obtained by the joint test kit,the statistical relationship between the baselines of the two different pathogens was analyzed based on the baseline of the patients and the normal persons,the gender of the patients,the age of the patients,and the types of diseases.The statistical results showed that between the patient and the healthy control group,the p values of Uu and Up are both less than 0.05.Pathogen Uu is much more in males(26/48,54.17%),while Up is the main body infection is in female(230/273,84.25%).But there is no obvious correlation between the infection of these two pathogens and the different age groups between men and/or women(p>0.05).In the statistical analysis of men’s diseases,the p values of the two pathogens Uu and Up are both less than 0.05,which meaned that the male patient samples collected were all involved with inflammation,infertility,and other three major types of diseases.However,in the disease classification of female patients,the infection of Uu was not statistically significant(p>0.05),while the p value of Up was less than 0.05,which indicated that Up was the specific pathogen of infection in one or several kinds of diseases of women.Then,using different genders and different diseases to establish a regression model for analysis,it was found that there was a statistical relationship between Up and male infertility and female infertility(p<0.05).Bacterial vaginosis(BV) is a common disease of female genitourinary tract.In this study,we also analyzed the correlation between these two pathogens and female bacterial vaginosis.Among the 273 female samples,a total of 224 cases have completed the bacterial vaginosis test,and the p value in the statistical analysis of the correlation between BV and Uu/Up is greater than 0.05,indicating that both Uu and Up are not related to BV infection.These results provided certain evidence for the study of the pathogenesis of Ureaplasma Urealyticum and Ureaplasma parvum,as well as a certain reference basis for the necessity of clinical detection of these two types of pathogens.