Functionalized Ordered Mesoporous Carbon Nanosystems For NIR Responsive Chemo-photothermal Therapy Of Cancer Cell

With the development of nanomaterials in the field of cancer treatment,the nanomaterials have opened up a new way for the treatment of tumor,the construction effective nanoparticle drug delivery system is of great significance for cancer therapy.Chemotherapeutic drugs have greater toxic and side effects when directly applied to tumor treatment,this problem can be greatly avoided after they are loaded in nanomaterials.Because this method can effectively avoid early release of drugs to increase the drugs concentration in tumors,thereby reducing toxic and side effects.Moreover,drugs loaded in nanomaterials can not only effectively carry out chemotherapy,but also be applied to add other therapies for tumors.The shortcomings caused by single treatment model can be effectively solved.Ordered mesoporous carbon(OMC)is a promising component in drug carriers.It not only has the advantages of mesoporous materials such as tailorable mesoporous size,large specific surface area,diversity in surface modification,and low toxicity,but also has its own superior photothermal conversion ability.Using OMC as a carrier can solve the problems of complicated synthesis process,inefficient photothermal effects and hindered NIRmediated drug release caused by carrier who have not light to heat conversion capabilities when used for chemo-photothermal therapy of tumors.Based on the above advantages,this paper aims to construct a new ordered mesoporous carbon drug carrier for cancer cells treatment.We developed a drug delivery component based on OMC nanomaterials for NIR light triggered drug release and synergistic chemo-photothermal treatment for cancer cells.Mainly carried out the following two tasks:1.NIR-triggered drug delivery system based on phospholipid coated ordered mesoporous carbon for synergistic chemo-photothermal therapy of cancer cells.Chemo-photothermal treatment is one of the most efficient strategies for cancer therapy.However,traditional drug carriers without near-infrared absorption capacity need to be loaded with materials behaving photothermal properties,as it results in complicated synthesis process,inefficient photothermal effects and hindered NIRmediated drug release.Herein we report a facile synthesis of a polyethylene glycol(PEG)linked liposome(PEG-liposomes)coated doxorubicin(DOX)-loaded ordered mesoporous carbon(OMC)nanocomponents(PEG-LIP@OMC/DOX)by simply sonicating DOX and OMC in PEG-liposomes suspensions.The as-obtained PEGLIP@OMC/DOX exhibits a nanoscale size(600 ± 15 nm),a negative surface potential(-36.70 m V),high drug loading(131.590 mg/g OMC),and excellent photothermal properties.The PEG-LIP@OMC/DOX can deliver loaded DOX to human MCF-7 breast cancer cells(MCF-7)and the cell toxicity viability shows that DOX unloaded PEGLIP@OMC has no cytotoxicity,confirming the PEG-LIP@OMC itself has excellent biocompatibility.The NIR-triggered release studies demonstrate that this NIRresponsive drug delivery system enables on-demand drug release.Furthermore,cell viability results using human MCF-7 cells demonstrated that the combination of NIRbased hyperthermal therapy and triggered chemotherapy can provide higher therapeutic efficacy than respective monotherapies.With these excellent features,we believe that this phospholipid coating based multifunctional delivery system strategy should promote the application of OMC in nanomedical applications.2.NIR-triggered drug delivery system based on DNA functionalized ordered mesoporous carbon.Combined with the situation of the previous work,which has a problem with releasing drug too quickly,we here further develop a DNA-functionalized and DOXloaded OMC nanocomponent for NIR-triggered drug delivery.In this section,OMC/DOX complex was first synthesized by incubating them together.After DOX loading,a DNA(Tm = 51.3 ℃)with hairpin structure was subsequently grafted on the surface of OMC through the electrostatic adsorption between DNA and OMC.And then,this multifunctional system was finally obtained(DNA-OMC/DOX).Under the irradiation of NIR,OMC can effectively convert NIR laser into heat,leading to the damage of hairpin structure and the release of loaded DOX.However,when the NIR laser was closed,the hairpin structure of DNA has reformed by the decreased temperature,resulting in the close of pores.With the alternative use of NIR laser,the reversible controlled release of drug can be finally implemented.The experimental results show that this system has a good reversible controlled release behavior under NIR light stimulation.We believe that DNA-OMC/DOX can provide new ideas for the development of multifunctional nano drug carriers,and it can further promote the research of OMC for synergistic chemo-photothermal therapy of cancer cells.