The Construction And Application Of ABO Blood Group Gene Typing Technology Based On Single Nucleotide Polymorphism

In clinical medicine,blood transfusion is not only a routine treatment but also an emergency treatment.As an important part of modern medicine,blood transfusion saves the lives of countless people and provides strong support for emergency treatment of trauma,organ transplantation,tumor high-dose chemotherapy,blood Medical treatments such as systemic disease treatment and major surgery.At present,the ABO blood group system is the first system discovered by mankind.Its discovery opened a new era of immunohematology research and laid the foundation for scientific blood transfusion.The ABO blood group system is of great significance in the fields of genetic research,clinical blood transfusion,and transplantation immunity.At present,the clinical identification of ABO blood type mainly depends on the serological method which is relative cheap,rapid and standardized.However,the agglutination intensity of antigens and antibodies will change in different subtypes,resulting in missed detection or misconceptions,which is likely to cause severe transfusion reaction.The genetic testing is an effective alternative method to make up for the lack of serological testing methods.With the continuous development of molecular biology technology and its application in blood group research,the molecular biology mechanisms of almost all known blood group systems have been elucidated.Since then,a variety of genotyping technologies have been applied to ABO blood group typing.Genotyping technology can achieve perfect matching between patient and donor blood groups,and to build a reliable ABO blood group genotyping for this purpose Type method is of great significance.In this study,we successfully constructed a method based on SNa Pshot technology for ABO blood group subtyping.20 SNP loci were typed through this method,including the third intron(ISV3 + 5),sixth exon(261,297),seventh exon(407,425,467,526,559,640,641,695,700,ABO gene(703,761,803,810,871,892,1054,1061).Then,28 ABO alleles were typed according to the typing results,and the method was optimized during the experiment.Using this method,the ABO blood type of 146 unrelated individuals’ peripheral blood samples was identified,and 12 alleles(A101,A102,B01,B303,B305,Bw11,O101,O102,O02,B(A)02,B(A)04,Cis AB01),23 different genotypes(6 types of A are A102 / A102,A101 / A102,A101 / O01,A101 / O102,A102 / O01 and A102 / O102;7 types of B type are B01 / B01,B01 / O01,B01 / 102,B305 / O01,B305 / O102,Bw11 / O01 and Bw11 / O02;4 types of AB type are A101 / B01,A101 / B305,A102 / B01 and A102 / B303;three types of O type are O01 / O01,O01 / O102 and O102 / O102;three types of rare subtypes are B(A)02 / O01,B(A)04 / O102 and Cis AB01 / O2)were successfully identified and the accuracy rate reaches 100%,which verifies the accuracy and repeatability of the method and provides a reliable method for ABO blood group subtyping.