Clinical-acupoint Rule And Transcriptome Analysis Of Jin’s Three-needle Technique In The Treatment Of Mental Retardation

ObjectiveMental retardation is one of the most dangerous diseases among children with the highest rate of disability,which causes a heavy burden on families and society.The etiology of mental retardation is complex,there are many patients with unclear etiology,and the clinical misdiagnosis.rate is high.Jin sanacupuncture is a commonly used method for the clinical treatment of mental retardation.Its effect has been verified in clinical practice and basic scientific research for many years,and it has been rated as an appropriate technology for mental retardation by the national administration of traditional Chinese medicine.In order to explore the characteristics and potential biological characteristics of the diagnosis and treatment of mental retardation,two studies are proposed.To investigate the potential biological characteristics,signaling pathways and related candidate genes of mental retardation based on whole blood rna-seq.It provides biological information for the etiology of mental retardation and scientific basis for the improvement of clinical diagnosis and treatment.MethodsTo collect and consult the cases of the first affiliated hospital of guangzhou university of traditional Chinese medicine,outpatient department of acupuncture and XX traditional Chinese medicine clinic,and to collect and summarize the case information of the children with mental retardation treated with jin’s three-needle therapy from October 2017 to October 2019.The Excel 2013 software was used to establish the case data collection table,and the symptoms and acupoint information were entered into the case data collection table.The cases that did not give clear symptoms,acupoint information or other basic collection information were excluded,and the corresponding database was established.To "practical Chinese medicine diagnostics（second edition）","practical child encephalopathy（third edition）","jin sanji.Act for the standard to carry on the data information normalization processing.Mainly using software SPSS20 and R language,descriptive analysis,association rule analysis and cluster analysis were conducted on the information of symptoms and acupoints.Select into standard developmental disorder children,state of health of six children,six disease group and normal group,respectively,the extraction 2 ml whole blood,TRIzol method is used to extract total RNA in the organization,and use the software fastx_toolkit₀.0.14 to detect the quality of RNA samples,after passing a DNA library construction and sequencing,the Cufflinks software is adopted to transcriptome data assembly and splicing,USES software to analyze differentially expressed genes between groups DESeq2,Will receive the transcriptome assembly of all genes and transcription and public database（NR,Swiss Prot,Pfam,EggNOG,GO and KEGG）compare,obtain the function of gene transcription and the comprehensive information and statistical database for each database annotation situation to compare,obtain the function of developmentally disabled children differentially expressed gene’s information,GO to function classification and KEGG pathway analysis,screening candidate genes that may be relevant.ResultsA total of 441 valid medical records were collected and included in the analysis,among which 106 cases were involved,with a total of 2498 symptom occurrence frequency and 109 acupuncture points/groups.The highest symptom occurrence frequency was 5201 in total,with a total of 192 cases and a frequency of 7.69%.The second is poor comprehension,with frequency of 188 and frequency of 7.53%.The acupoint group with the highest frequency of use was the three cerebral needles,with a total of 432 times and a frequency of 8.31%.Followed by four anchor,use frequency is 423,the frequency of 8.13%using the association rules analysis further symptoms of article 15 of association rules,it is concluded that 14 holes for association rules.The results of clustering analysis,a total of six meridians clustering party c DNA library sequencing results were produced（50.16 3.52）（49.55 6.55）of article 106 of the 106 reads,after filtering treatment to get（49.34 3.45）（48.75 6.47）of article 106 of the 106 reads,the clean each sampleOn the outside of the 6 Gb to sample the GC content is between 53.61%～58.10%,Q20 base content is between 98.20%～98.63%,Q30 bases were greater than 94%（see table 4）,according to data sequence are of good quality and high credibility by the GO database annotations,classification to the biological process of 226 genes,form cell components related genes have 134,participate in the molecular function（molecu lar function）of 41The main enriched pathways to KEGG differentially expressed genes were Alanine,aspartate and glutamate Glycine,Serine and threonine metabolism belong to the metabolism pathway,and pi3k-akt signaling pathway nf-kappa B signaling pathway belongs to the Environmental Information Processing pathway,Autophagy apoptosis-multipleSpecies belongs to the Cellular Processes pathway,the Intestinal immune network for IgA production belongs to the Organismal Systems pathway,and the epstein-barr virus infection Influenza A in Primary immunodeficiency belongs to the human disease pathwayFour possible related candidate genes were identified,ASPA、GAD、DNM1L、CAD.ConclusionDevelopmental disorder children’ s clinical symptoms to the most common language barrier movement disorders mental ability three aspects,the part is associated with sleep disorders and problem behaviors such as jin’s 3 needling in the treatment process,extensive use of head acupuncture point group directly stimulate the pathological changes of the brain,the brain use the largest number three stitches,the other with sufficient intelligence needle "ba liao" tonic to the deficiency of kidney to compensate,1r 3 li4 foot three stitches of spleen and regulate the day after tomorrow to supplement qi and blood.