| Objective Cancer is a major public problem in the world.Colon cancer is the most common fatal cancer in the digestive system.At present,chemotherapy is the main treatment for patients with advanced colorectal cancer.However,due to the improvement of drug resistance of tumor cells and the side effects of chemotherapy,the treatment effect is not satisfied,and the survival rate of patients is low,so it is urgent to find a new way.Because of its small side effects and wide application,magnetic field is expected to become a new way of tumor treatment.At present,clinical studies have shown that magnetic therapy plays an active role in cancer treatment.Ferroptosis is a kind of cell death mode proposed in recent years.It depends on the existence of iron and the accumulation of reactive oxygen species,which has something in common with the theory of reactive oxygen species of magnetic field.Therefore,whether magnetic field can enhance ferroptosis and its mechanism is worth exploring.The purpose of this study is to study the effect of low intensity static magnetic field on ferroptosis of colon cancer cells,and to reveal the possible molecular mechanism.Methods Erastin was used to treat colon cancer cells with a certain concentration of 10μmol/L,20μmol/L and 30μmol/L for 48 hours.The ferroptosis indexes(iron,MDA,GSH and GPX)were measured to select one best concentration.After 12,24,48 and 72 hours,the best intervention time was selected by measuring the ferroptosis indexes.Erastin was used as ferroptosis inducer and ferrostatin-1 was used as ferroptosis inhibitor.After 48 hours of intervention with Erastin /ferrostatin-1and magnetic field,GSH,MDA,iron content and GPX vitality were detected by biochemical kit.CCK8 was used to detect cell proliferation ability,Transwell test was used to detect cell migration and invasion ability,FITC / PI staining was used to detect apoptosis rate.RT-PCR was used to detect the difference of mi R-28-5p and N4BP1 expression between HCT116,SW480 and NCM460.After 48 hours of intervention with Erastin and magnetic field,the expression of mi R-28-5p and N4BP1 in HCT116 cells was detected by RT-PCR,and the protein level of N4BP1 was detected by Western blotting.Transfect mi R-28-5p mimics and mi R-28-5p inhibitors into cells with ferroptosis to study the effects of mi R-28-5p on cell proliferation,migration,invasion ability and apoptosis.RT-PCR and Western blotting were used to detect the expression of N4BP1.After mi R-28-5p was transfected,Iron,MDA,GSH content and GPX vitality were measured to study the effect of mi R-28-5p on ferroptosis.Results The effect of Erastin on ferroptosis increased with time and concentration.After the intervention of Erastin,the iron content and MDA content increased in the cells,GSH content and GPX vitality decreased,especially after the addition of magnetic field.The above changes disappeared after the addition of inhibitors.Cell proliferation,migration and invasion ability were weakened after ferroptosis,but apoptosis did not change significantly.The expression of mi R-28-5p in HCT116 and SW480 cells was lower than that in NCM460 cells.The expression of mi R-28-5p in HCT116 and SW480 cells was higher than that in NCM460 cells.When HCT116 cells was intervention by Erastin,the expression of mi R-28-5p increased,while that of N4BP1 decreased.The increase of mi R-28-5p expression and the decrease of N4BP1 expression were more significant after adding magnetic field.Overexpression of mi R-28-5p can enhance ferroptosis induced by Erastin,and weaken the proliferation,migration,invasion ability of colon cancer cells,but enhance apoptosis,while silencing mi R-28-5p had the opposite effect.Overexpression of mi R-28-5p can decrease the expression of N4BP1 and enhance the ferroptosis induced by Erastin,while silencing mi R-28-5p had the opposite effect.Conclusions Magnetic field played an important role in promoting ferroptosis of colon cancer cells,which may be due to the negative regulation of N4BP1 by mi R-28-5p. |
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