The Protective Effect Of Astragaloside A On Cerebral Ischemia-reperfusion Injury And Its Related Mechanism

objectiveTo investigate the effects of astragaloside IV on the degree of cerebral infarction after focal cerebral ischemia-reperfusion injury in rats and the changes of Caspase-3,Caspase-9 and Bcl-2 protein expression on the cerebral ischemic side.Methods75 healthy male SD rats(SPF grade)were selected,weighing 250 g ± 20 g,and randomly divided into sham operation group(Sham),model group(Model),dimethyl sulfoxide vehicle group(DMSO)and astragaloside IV(AS-IV),Edaravone group,a total of five groups,15 rats in each group.The model of middle cerebral artery embolization(MCAO)in rats was established with reference to Longa et al.Except for the sham operation group,the other groups were embolized for 2 hours.Astragaloside group was intraperitoneally injected at 12 h after reperfusion,edaravone group was intraperitoneally injected at 1h and 3h after reperfusion,the other groups were intraperitoneally injected at 12 h after reperfusion.After 2 hours of embolization and 24 hours of reperfusion,each group was referred to the Zea longa scoring standard for neurological behavioral scoring.The reliability of model preparation was evaluated,the TTC staining method was used to determine whether the model was successfully made,the volume ratio of cerebral infarction(%)was measured,and the brain tissue pathology was observed by HE staining.The expression of Caspase-3,Caspase-9 and Bcl-2 was detected by immunohistochemistry and Western Blot.ResultsFrom a behavioral point of view,After 24 hours of reperfusion the rats in the Sham group had no obvious neurological deficit symptoms,and their biological behavior score was 0 points.The other four groups had different degrees of neurological deficit symptoms.The degree of neurological deficits in the rats in the group was more significant than that in the astragaloside group and the edaravone group(P<0.05).TTC staining showed that the infarcted areas of different degrees appeared in all groups except the Sham group.Compared with the Model group,the infarct volume of the astragaloside group and the edaravone group decreased(P<0.05).The results of immunohistochemistry and immunoblotting showed that Caspase-3,Caspase-9,and Bcl-2 were expressed after cerebral ischemia and reperfusion.Compared with sham operation group,Caspase-3 and Caspase-9 were expressed on the ischemic side of the brain in the Model and DMSO groups.But compared with the Model group,the expression of the Astragaloside group and the Edaravone group was significantly reduced(P<0.05).Compared with the Model group,the expression of Bcl-2 in the ischemic side of the model group and the DMSO group was reduced,but compared with the model group,the expression of astragaloside and edaravone group was significantly increased(P<0.05).ConclusionsAstragaloside IV can reduce cerebral infarction area after cerebral ischemia-reperfusion injury.And it can inhibit the apoptosis by inhibiting the expression of the Caspase-3,Caspase-9 and the up-regulation of Bcl-2 protein,and then exert the protective effect after cerebral ischemia-reperfusion injury.