| ObjectiveTo explore the effect of Berberine(BBR)on the expression of Glucose transporter 4(GLUT4)in myocardial tissue of Type 2 Diabetes(T2DM)rats and its protective effect on myocardial tissue.MethodsSelect 80 healthy SPF grade Sprague-Dawley(SD)male rats weighing between 280g-300 g and adopt High Fat-high Sucrose Diet(HFS).After one week of adaptive feeding,80 large rats were randomly divided into 4 groups for experiments:(1)control group(CON group),(2)model group(T2DM group),(3)T2DM + berberine treatment group(BBR group),(4)T2DM+metformin hydrochloride In the treatment group(MHT group),20 rats in each group.Rats in the CON group were injected intraperitoneally with citrate buffer(50mg / kg),and rats in the T2 DM,BBR,and MHT groups were injected intraperitoneally with streptozotocin(STZ)50mg / kg.STZ has antibacterial,antitumor and diabetic side effects.It has highly selective toxic effect on islet β cells,which leads to experimental diabetes.Three days later,If the fasting blood glucose(FBG)concentration of T2 DM,BBR and MHT group rats was ≥16.7mmol/L,and the appearance of polyphagia,polyuria and polydipsia was considered to be successful in diabetes modeling.After successful disease modeling,they were still raised by the above method,and the general state of rats was observed every day.After successful modeling,the CON group and the T2 DM group were given daily citrate buffer 50 mg / kg,the BBR group was given daily BBR 50 mg / kg in the morning,and the MHT group was given daily MHT 50 mg / kg in the morning,with continuous treatment 8w.The weight of rats in each group was measured before treatment,and the weight and FBG were measured at 2w,4w,6w,and 8w after treatment.The method was as follows: randomly select 5 rats in each group to measure body weight,and then collect blood at the tip of the tail to detect FBG.Before the measurement,rats in each group were fasted and deprived of water for 12 hours.At 8w,5 rats were randomly selected from each group for routine anesthesia.Hearts were removed for Periodate-Schiff reaction(PAS),Immunohistochemistry(IHC),and Western Blotting(WB)to detect the changes of carbohydrate in myocardial cells of each group.ResultsThe general state of the rats in the BBR group was significantly better than that in the T2 DM group.PAS showed that the pathological changes of the myocardial tissue in the BBR group were lighter,and the myocardial cells in the T2 DM group were significantly edema.Rise.ConclusionsBBR can enhance the expression of GLUT4 in myocardial cells of T2 DM rats,and has a significant hypoglycemic effect,which can reduce the degree of lesions in myocardial tissues of T2 DM rats. |
PDF Full Text Request