Expression Of IL-10 And IL-15 In Serum Of Patients With Multiple Myeloma

Objective:There is evidence that a cytokine network is implicated in controlling proliferation,progression and dissemination of human multiple myeloma(MM)cells.The purpose of this article is to study the relationship between interleukin-10(IL-10)and interleukin-15 in patients with multiple myeloma(MM)by measuring their concentration,in order to provide new ideas and evidences for the occurrence,development and treatment of MM.Methods:In this experiment,between April 2014 and February 2020,a total of 49 subjects who attended the Zhongshan Hospital affiliated to Xiamen University were tested.According to the inclusion criteria,a total of 14 untreated MM patients,20 MM patients who completely response after treatment were used as the experiment group,15 healthy individuals were used as controls.All subjects were collected with 5 milliliter serum samples,and stored at-80℃ for further evaluation.Enzyme-linked immunosorbent assay(ELISA)was used to detect whether the levels of IL-10 and IL-15 in the serum of all observation subjects were different in healthy control group and MM experimental group,and after treatment of MM patients,these two cytokines are there any differences in expression levels in serum.The expression levels of the two cytokines were calculated and analyzed statistically to see if they have statistical significance and diagnostic or predictive value.Results:1.Compared with the healthy control group,the expression levels of IL-10 and IL-15 in the serum of patients with untreated multiple myeloma was significantly higher than in the control group(P<0.001).2.The level of serum IL-10 and IL-15 in MM patients with stage Ⅱ and Ⅲ were significantly higher than those with stage I(P<0.05).There was no significant difference in the levels of IL-10 and IL-15 between patients with stage Ⅱ andⅢ(P<0.05).3.At different stages of the disease,the expression levels of IL-10 and IL-15 in the serum of MM patients were decreased after treatment(P<0.05).4.No significant correlation between IL-10 and IL-15 levels in MM patients’serum(R=0.527,P=0.053).Conclusions:Our study suggests that abnormal expression of cytokines IL-10 and IL-15 is one of the clinical features of myeloma.Monitoring serum IL-10 and IL-15 levels can help diagnose,stratification of disease and evaluation of treatment effect.