Effect Of Strontium Phosphosilicate Bioceramics On The Proliferation And Odontogenic/angiogenic Differentiation Of Human Dental Pulp Stem Cells

Objective:1.Using traditional β-tricalcium phosphate(β-TCP)as a control,search for the effect of Strontium Phosphosilicate bioceramics on the proliferation of hDPSCs,and screen out the appropriate extract concentration.2.To evaluate the effect of SPS on odontogenesis and angiogenesis of hDPSCs,and to explore new potential scaffolds for dental regeneration.Method:1.To obtain the primary cells of human dental pulp stem cells by modified tissue culture.2.The passage and expansion of human dental pulp stem cells.3.Preparation of SPS and β-TCP extract.4.MTT assay was used to detect the proliferation of hDPSCs by SPS and β-TCP extract,and then screen out the appropriate concentration.5.To detect the effect of SPS and β-TCP extract on odontogenic and angiogenic differentiation of hDPSCsc by alkaline phosphatase(ALP)activity assay.6.The effects of SPS and β-TCP extract on the expression of odontogenic and angiogenic genes in hDPSCs were detected by real-time quantitative polymerase chain reaction(RealTime-PCR).Result:1.Human dental pulp cells were isolated and cultured by tissue mass method,and after 3-5 generations,human dental pulp stem cells with high purity were obtained.2.SPS extract can promote the proliferation of human dental pulp stem cells,the proliferation of hDPSCs which cultured in 25mg/ml,12.5mg/ml,6.25 mg/ml concentration of SPS extract is significant.3.SPS bioceramic extract can early promote the hDPSCs to odontogenic and angiogenic.The quantitative and qualitative staining of ALP of hDPSCs which cultured in 12.5 mg/ml concentration of SPS extract is significant after 10 days.The mRNA expression of odontogenic and angiogenic gene is also increased in early time,with time-dependent.Conclusions:12.5 mg/ml SPS bioceramic extract can significantly promote the proliferation of hDPSCs,increase the expression of odontoblasts and angiogenesis-related genes,and promote the differentiation of hDPSCs into odontoblasts and blood vessels.