The Function And The Mechanism Of OGD-induced EPC Transfected With MicroRNA-210-3p

Objectives: The expression of microRNA-210-3p was up-regulated in endothelial progenitor cells,but its effect on the function of endothelial progenitor cells is not clear.In order to explore the possible effects and targets,we up-expressed or downexpressed microRNA-210-3p in endothelial progenitor cells induced by oxygen and glucose deprivation.Methods: Endothelial progenitor cells was extracted from umbilical cord blood of the healthy parturient women.We transfected mimic and inhibitor of microRNA-210-3p into endothelial progenitor cells,and after 24h,we performed them a model of oxygen and glucose deprivation for 4h.We detected the level of microRNA-210-3p by RTPCR.and tested the change of proliferation,tubeformation,migration of OGD 4h-induced EPCs by CCK-8,matrigel and transwell.Using mi RWalk targets library,we chose RGMA as a direct target of microRNA-210-3p to explore the underlying mechanisms.We detected the change of RGMA in OGD 4h-induced EPCs after up-or down-expressed microRNA-210-3p in m RNA and protein level by RT-PCR and Western Blot.Dualluciferase reporter system was used to verify whether RGMA was the direct target of microRNA-210-3p.Micro RNA-210-3p might regulate the function of OGD-induced EPCs through RGMA.Results:1.Overexpression of microRNA-210-3p in OGD-induced EPCs could promote its abilities to proliferation,tubeformation,migration(p < 0.05)and vice versa(p < 0.05).2.Overexpression of microRNA-210-3p in OGD-induced EPCs could inhibit the expression of RGMA(p < 0.05),whereas facilitate(p < 0.05).3.Dual-luciferase reporter system proved RGMA was the direct target of microRNA-210-3p.Conclusions: MicroRNA-210-3p enhanced the function of OGD-induced EPCs in angiogenesis.And microRNA-210-3p might be involved in the process through RGMA.