The Effect Of Method Of Tongfu Xingshen To The Endogenous Neural Stem Cells In Experimental ICH Rats

ObjectiveBy observing the proliferation and differentiation of neural stem cells in the lateral ventricle of rats with experimental cerebral hemorrhage and the expression of related genes in this process,the effect of Tongfu Xingshen method on the proliferation and differentiation of neural stem cells was explored,and the mechanism of promoting the proliferation and differentiation of neural stem cells in the subventricular area of cerebral hemorrhage and the repair of injury was discussed.MethodsIn this study,SD rats were selected to reproduce cerebral hemorrhage model by autologous blood injection.The changes of neurological deficit,edema and EB content in experimental cerebral hemorrhage rats were observed on the 1st,7th,14th and 28th day after the model was established.The changes of endogenous proliferation and differentiation in SVZ area of experimental cerebral hemorrhage rats under the influence of Tongfu Xingshen method were observed.The effect of Tongfu Xingshen method on the expression of BDNF mRNA and TrkB mRNA genes induced by proliferation and differentiation were further observed.Results(1)Neurological function scores:7 days group,14 days group,28 days group,compared with the model group,the scores of the high concentration group and the low concentration group were decreased,the difference was statistically significant(P<0.05,P<0.05).(2)EB content:1 day group,7 days group,14 groups,compared with the sham operation group,the EB content in the model group increased,the difference was statistically significant(P<0.01,P<0.01,P<0.01);In the 7-day group,compared with the model group,the EB content in the high concentration group and the low concentration group decreased,the difference was statistically significant(P<0.05,P<0.05).In the 14-day group,compared with the model group,the EB content in the high concentration group decreased,and the difference was statistically significant(P<0.05).(3)Brain water content:1 day group,7 days group,14 days group,compared with the sham operation group,the brain water content of the model group increased,the difference was statistically significant(P<0.01,P<0.01,P<0.05).In the 7-day group and the 14-day group,compared with the model group,the brain water content in the right posterior region of the high-concentration group and the low-concentration group decreased,and the difference was statistically significant(P<0.05,P<0.05).(4)The number of BrdU single-label positive cells:7 days group,14 days group,28 days group,compared with the sham operation group,the number of BrdU positive cells in the model group increased,the difference was statistically significant(P<0.01,P<0.01,P<0.01);In the 14-day group,compared with the model group,the number of BrdU-positive cells in the high-concentration group increased,the difference was statistically significant(P<0.05);in the 28-day group,compared with the model group,the high concentration group and the low concentration The number of BrdU positive cells increased,and the difference was statistically significant(P<0.01,P<0.01).(5)Nestin/BrdU double-label positive cells:In the 7-day group,compared with the sham operation group,the number of Nestin/BrdU double-label positive cells in the model group increased,the difference was statistically significant(P<0.01).Compared with the model group,the number of Nestin/BrdU double-labeled positive cells in the high concentration group and the low concentration group increased,the difference was statistically significant(P<0.01,P<0.01).(6)NeuN/BrdU double-label positive cells:14 days,28 days,compared with the sham operation group,there was no significant difference in NeuN/BrdU double-labeled cells in the model group(P>0.05,P>0.05).There was no significant difference in the number of NeuN/BrdU double-labeled positive cells between the high-concentration group and the low-concentration group in the 14-day group and the 28-day group.The difference was not statistically significant(P>0.05,P>0.05).(7)GFAP/BrdU double-label positive cells:14-day group,28-day group,compared with the sham operation group,the model group GFAP/BrdU double-label positive cells increased,the difference was statistically significant(P<0.01,P<0.01);14 days group,28 days group,compared with the model group,the number of GFAP/BrdU double-label positive cells in the high concentration group and the low concentration group increased,the difference was statistically significant(P<0.01,P<0.05).(8)BDNF mRNA expression:In the 7-day group,compared with the sham operation group,the expression of BDNF mRNA in the model group increased,the difference was statistically significant(P<0.01);compared with the model group,the high concentration group and the low concentration group The expression of BDNF mRNA was increased,and the difference was statistically significant(P<0.01,P<0.05).Compared with the model group,the expression of BDNF mRNA in the high concentration group was significantly increased in the 14-day group,and the difference was statistically significant(P<0.01).(9)TrkB mRNA expression:In the 7-day group,compared with the sham-operated group,the expression of BDNF mRNA in the model group increased,the difference was statistically significant(P<0.05).Compared with the model group,the TrkB mRNA expression in the high-concentration group The difference was statistically significant(P<0.01).In the 28-day group,the expression of TrkB mRNA was increased in the high-concentration group,which was statistically significant compared with the model group(P<0.01).ConclusionThrough the experimental study,it is proved that the application of "Tongfu Xingshen"method has a significant effect on improving the neurological deficit of rats with cerebral hemorrhage,reducing brain water content and promoting the permeability repair of blood-brain barrier:the successful induction of Proliferation and differentiation of endogenous NSCs in the subventricular zone participates in the repair of neurological function.From the gene level,it is possible to promote the increase of BDNF mRNA and TrkB mRNA in the subventricular region of the lateral ventricle,thereby promoting the proliferation and differentiation of endogenous NSCs and particiPating in the repair of the lesion.