Application Of Ultrasonic Diagnosis In Monitoring The Size Of Tumors In Mouse Treated Using Doxorubicin-loaded Graphene

Chemotherapy,as an important treatment for cancer,not only kills tumor cells but also damages normal cells,resulting in toxic and side effects on patients.As a new two-dimensional carbon nanomaterial,graphene has attracted great attention of medical scientists in recent years.Because of its large specific surface area and easy surface modification,the research on drug carriers is developing rapidly.In this paper,we will carry out the research on graphene-loaded doxorubicin in the treatment of cancer.In the past,the detection of tumor therapeutic effect is to execute animal samples and collect tumor analysis,which is costly,time-consuming and more important,it is impossible to avoid individual differences in animals.Therefore,this study attempts to apply ultrasound diagnosis to real-time,in situ detection of tumor therapeutic effect.In this study,the graphene particles were characterized by uniform particle size and large specific surface area.The loading rate of doxorubicin on the surface of graphene was 300%.Infrared spectrum confirmed that doxorubicin was successfully loaded on the surface of graphene.High frequency ultrasound and shear wave elastography were successfully used to trace,image,accurately measure the size and Young’s modulus of tumor in mice.The tumor model of mice was successfully constructed by subaxillary injection of tumor cells.When the initial tumor size was comparable,the tumor-bearing mice were treated with two treatment schemes of direct administration and injection of doxorubicin-loaded graphene.The data of tumor growth size and Young’s modulus in situ diagnosed by ultrasound suggested that compared with the control group and the doxorubicin group,the animals in the graphene group were significantly prolonged the survival time.Further histological analysis of the tumor showed that the graphene-loaded group had the greatest destructive power to the tumor cells,so that the treatment effect was the best.In this paper,we studied the effect of graphene as a targeting drug carrier on tumor treatment.The changes of tumor in mice were tracked in situ by ultrasonic diagnostic method.The advantages of graphene as a drug carrier were confirmed by combining with Young’s modulus and other indicators,which provided a theoretical basis for the application of targeted therapy for tumor.