Expression Of IL-6 And PRL In Breast Cancer And Its Clinical Significance

Background:Breast cancer is the most common tumor diagnosed by women in the world.It is also the leading cause of female cancer death.Every year,more than 1 in 10 cancers are diagnosed as breast cancer.The most common pathological type of breast cancer is invasive ductal cancer,accounting for about 60%to 75%of breast cancer.Worldwide,the five-year prevalence rate of breast cancer shows that about 11%of the world’s breast cancer occurs in China,and the incidence has rapidly increased in recent decades.Compared with Western countries,the 5-year survival rate of breast cancer patients in China is still relatively low(73.1%),especially in remote areas(55.9%).The death rate of breast cancer in China accounts for about 10%of the deaths of breast cancer in the world.About 90%of the deaths are due to distant metastases.Early diagnosis and treatment can significantly improve the survival rate,but breast cancer The cause of prevention has always been the goal of clinical pursuit.In recent years,inflammation and tumor have become the focus of tumor etiology.In the process of tumorigenesis and development,interleukin-6(IL-6)and other inflammatory factors can make tumor cells and their tumor microenvironment.Weave into a network to promote the occurrence and progression of tumors;IL-6 is the most important inflammatory factor that binds inflammation and tumors.It is involved in innate immunity,adaptive immunity,acute and chronic inflammation,and tumor pathogenesis and development.Play a vital role.On the other hand,as a heterogeneous disease,breast cancer includes risk factors such as fertility factors,key menstrual periods and estrogen exposure,mental stress,diet and obesity,unhealthy lifestyle,and prolactin(PRL)as a risk factor.A class of hormones that are regulated by behaviors such as stress,sleep,unhealthy life,and high-nutrient fat diets are also risk factors for breast cancer;IL-6 and PRL have been proven by domestic and foreign literature to be highly expressed in breast cancer serum.And it is related to the poor prognosis of the patient.Foreign researchers in the study of patients with systemic lupus erythematosus found that elevated PRL can increase the secretion of IL-6 from peripheral blood mononuclear cells in patients with systemic lupus erythematosus;currently,PRL and inflammatory factor IL-regulated by behavioral factors 6 There is little research on the expression and relationship between breast invasive ductal carcinoma and paracancerous lesions.The study of PRL and IL-6 expression and correlation may reveal the intrinsic link between behavioral factors,hormones regulated by behavioral factors and internal environment inflammation,which is conducive to the search for targeted preventive measures and prognostic markers.Objective:1.To study the expression of PRL and inflammatory cytokine IL-6 in breast invasive ductal carcinoma,paracancerous,fibroadenoma and fibroadenoma tissues,and to explore the expression and progression of breast invasive ductal carcinoma.relationship;2.To investigate the correlation between PRL and inflammatory cytokine IL-6 expression in breast tissue and to provide a theoretical basis for exploring the interaction between PRL and IL-6.Methods:From 2013 to 20 1 7,63 patients with breast invasive ductal carcinoma who underwent general surgery in our hospital and 17 patients with breast fibroadenoma at the same period,the pathological data and follow-up information of the two groups were complete.Immunohistochemistry was used separately.To detect the expression of PRL and IL-6 in breast infiltrating ductal carcinoma tissue,adjacent normal tissue,breast fibroadenoma and breast fibroma tissue,and to collect age,sex,tumor size,histological type and TNM staging.,Lymph node metastasis,ER expression,PR expression,Her-2 and other related clinicopathological parameters,and the survival prognoses of 63 patients with infiltrating ductal breast cancer were followed up.The expression of PRL and IL-6 in breast infiltrating ductal carcinoma was analyzed.Clinicopathological features and the relationship between survival and prognosis;the correlation between behavior-regulated PRL and inflammatory factor IL-6 expression in patients with invasive ductal carcinoma of the breast;ResultThe average age of 63 invasive ductal carcinomas of the breast was 51 years old(28 to 70 years old),the median age was 51 years,and the follow-up deadline was February 2018.At the time of follow-up,4 patients died and 10 patients relapsed.59 Name survives.Firstly,IL-6 was detected in infiltrating ductal carcinoma tissue,paracancerous tissue,breast fibroadenoma,and paratumor tissue from routine postoperative pathological sections.The positive rate of interleukin-6 in cancer tissue was 82.5.%(52/63),paraneoplastic tissue positive rate was 31.7%(20/63),breast fibroadenoma tissue positive rate was 41.2%(7/17),fibroma paraneoplastic positive rate was 5.88%(1/17);The positive rate of IL-6 in breast infiltrating ductal carcinoma was higher than that in the adjacent tissue(χ2=33.185,P<0.001)and breast fibroid tissue(P=0.016).PRL also showed similar results,in which the positive rate of PRL in cancer tissue was 81.0%(51/63),the positive rate in para-tumor tissue was 38.1%(24/63),and the positive expression in breast fibroadenoma tissues was positive.The rate was 41.2%(7/17),and the positive rate of expression in fibromas was 5.88%(1/17).The positive rate of PRL in breast infiltrating ductal carcinoma was also higher than that in the adjacent tissue(χ2=24.014,P<0.001)and breast fibroid tissue(P=0.034).Secondly,the expression of IL-6 and PRL in breast invasive ductal carcinoma combined with pathological parameters analysis showed that the expression of IL-6 in invasive ductal carcinoma was related to tumor diameter(P=0.022)and TNM staging(P=0.003).There was no significant correlation with lymph node metastasis(P=0.115),histological grade(P=0.275),ER expression(P=0.312),PR expression(P=0.433),and Her-2 expression(P=0.675);PRL Similar expression was also observed in breast invasive ductal carcinoma.The expression of PRL in invasive ductal carcinoma was correlated with tumor diameter(P=0.036)and TNM staging(P=0.048),but with lymph node metastasis(P=0.100),histological grade(P=0.780),ER expression(P=0.179),PR expression(P=0.895),and Her-2 expression(P=0.549)were not significantly related;again,event-free survival analysis showed that The event-free survival time was lower in the IL-6 low expression group than in the high expression group(Log-rank=4.46,P=0.035),and the PRL also showed a longer event-free survival in the low expression group(Log-rank=5.00,P=0.025);Again,analysis of Cox’s univariate risk regression model revealed that IL-6 expression(P=0.041),PRL expression(P=0.033),lymph node metastasis(P=0.041)in cancer tissues,TNM staging(P<0.001)all affected the event-free survival of patients.Finally,the expression of PRL and IL-6 was higher in para-tumor tissues than in fibroid adenomas(P>0.05);the difference was statistically significant(IL-6,P=0.033;PRL,P=0.016);Spearman correlation analysis showed that PRL There was a significant positive correlation with IL-6 expression in breast invasive ductal carcinoma(rs=0.842,P<0.001).There was also a significant positive correlation between paratumor IL-6 and PRL expression(rs=0.842,P<0.001)Conclusion1.The high expression of PRL and IL-6 in breast infiltrating ductal carcinoma is related to the poor prognosis of patients.2.In this study,there was a significant positive correlation between the expression of PRL and IL-6 in breast infiltrating ductal carcinoma tissues and para-carcinoma tissues,while PRL was associated with behavioral factors such as sleep and high nutrient fat diets.Further study of PRL and IL-6 was conducted.The relationship may provide a theoretical basis for revealing the intrinsic link between behavioral factors,hormones regulated by behavioral factors and internal environment inflammation,and is conducive to proposing preventive measures for breast cancer etiology for behavioral factors.