The Research Of Overexpressing GAPDH Protects PINK1^B9 Transgenic Drosophila

Objective:To study the effect of transgenic Drosophila melanogaster on the transgenic Drosophila melanogaster model,and to explore the role of GAPDH in the model group after transgenic fruit flies.Methods:MHC-GAL4 was used as the promoter,and the normal fruit flies of MHC-GAL4 were obtained by hybridization with w¹¹⁸.MHC-GAL4 was hybridized with PINK1B9 as the experimental control group.MHC-GAL4/UAS system PINK1^B9 gene mutation PD transgenic Drosophila model,then PINK1^B9 gene Drosophila model hybridization with GAPDH overexpressing gene,intervention MHC-GAL4/UAS system PINK1^B9 system Transgenic Drosophila model will include MHC-GAL4 and w¹¹⁸ after hybridization MHC-GAL4 normal Drosophila together into the fifteenth day of wings morphological abnormalities statistics,flight ability detection,GAPDH m RNA detection,protein detection,muscle tissue ATP detection and electron microscopy to observe the successful construction of Drosophila model.Results:Compared with the normal Drosophila model,it was found that the wing rate of PINK1^B9 mutant PD transgenic Drosophila melanogaster was higher and the flying ability was decreased.The flying ability of the transgenic Daphne flora model was higher than that of the GAPDH gene,The mitochondrial morphology is improved.Conclusion:（1）GAPDH overexpression has protective effect on PINK1^B9 mutant transgenic Drosophila melanogaster model.（2）Overexpression of GAPDH may protect the neuroprotective effect of PINK1^B9 mutant PD transgenic Drosophila model by protecting the mitochondrial function to protect mitochondrial morphology.