Study On The Expression Levels Of MORC2 And NDRG1 Genes In Adipose Tissue Of High Fat-induced Obese Mice

Background and PurposeObesity is a chronic metabolic disease characterized by excessive increase in number and volume of adipocytes.It is closely related to the occurrence of many diseases such as cardio-cerebrovascular diseases and type II diabetes mellitus,and it is one of the global and crucial public health problems that damage human health.Our research group previously found that NDRG1 and MORC2 genes were differentially expressed in obese people through gene chip analysis.Moreover,consulting the existing literature suggest that NDRG1 and MORC2 genes were involved in various processes such as adipogenesis and tumorigenesis.Therefore,adipogenesis may be closely related to the occurrence of obesity.The purpose of this study is to detect the expression of NDRG1 and MORC2 genes in abdominal adipose tissue of high-fat induced obese mice,and to explore whether they could be potential targets for obesity therapy.MethodsTwenty-four C57BL/6 male mice were randomly divided into control group and obesity group.The mice in the control group were fed with normal diet,and the obese group were fed with high-fat diet for 24 weeks.The weight of mice was measured weekly.After successful modeling,blood samples were collected from control group and obesity group to detect serum total cholesterol(TC)and triglyceride(TG),abdominal adipose tissue was extracted,and real time polymerase chain reaction((Real Time Polymerase Chain Reaction,RT-PCR)was used to detect the expression levels of NDRG1 m RNA and MORC2 m RNA,and statistical methods were used to analyze all data finally.ResultsSerum TC in obese group were higher than those in control group(P<0.01),and TG in obese group were higher than those in control group(P<0.05).Both groups were statistically significant;The expression of NDRG1 m RNA in adipose tissue of obese group was higher than that of control group(P<0.01);The expression of MORC2 m RNA in adipose tissue of obese group was lower than that of control group(P<0.01),and the differences between the two results above were statistically significant.ConclusionsThe expression of NDRG1 m RNA in adipose tissue of obese mice increased,and the expression of MORC2 m RNA decreased.NDRG1 and MORC2 genes may be involved in the pathogenesis of obesity genes may be involved in the pathogenesis of obesity and are expected to be potential targets for obesity treatment.