Identification Of New Hub Genes For Clear Cell Renal Cell Carcinoma Through Bioinformatics Analysis

Background Clear cell renal cell carcinoma(cc RCC)is one of the leading causes of cancer-induced death worldwide.However,the molecular mechanisms of cc RCC remain unclearly.Methods In the present study,four messenger RNA microarray datasets(GSE36895,GSE46699,GSE53757,and GSE66272)were downloaded from the Gene Expression Omnibus(GEO)database.Differentially expressed genes(DEGs)between cc RCC tissues and adjacent kidney tissues were obtained from GEO2 R and the overlapping DEGs were identified.In addition,functional and pathway enrichment were performed by Funrich,while the protein–protein interaction(PPI)network construction were obtained from STRING and hub genes were visualized and identified by Cytoscape software.Furthermore,overall survival(OS)and tumor staging analysis of selected hub genes were performed by GEPIA.Results A total of overlapping 273DEGs(85 upregulated and 188 downregulated)were obtained.6 upregulated genes(LAPRM5,MS4A4 A,RGS1,MS4A7,LYZ and RNASET2)and 10 downregulated genes SCNN1 A,SLC12A3,WNK4,SCNN1 G,SLC34A1,HRG,SLC22A8,SLC22A6,AT P6V1B1 and SLC22A7)were Identified as hub genes of cc RCC.In addition,LYZ,RNASET2,SLC12A3,SCNN1 G,SLC34A1,SLC22A8,SLC22A6 and SLA22A7 are also related to the prognosis of patients and may be potential prognostic biomarkers.Conclusion In conclusion,current studies suggest that 16 genes may be key genes contributing to the development or progression of cc RCC,and further functional studies are needed to explore the mechanisms involved.