| Objective: To explore the intermittent swimming exercise with continued swimming mice with diabetes type Ⅱ sugar metabolism and the influence of the kidney,compared to intermittent swimming movement and swim for two ways which can better achieve intervention effect.Methods: 40 clean grade kunming male mice were randomly divided into 2 groups,8 in the quiet control group and 32 in the diabetes model group.After the establishment of the diabetes model,the group was divided into 3 groups: 8 in the quiet control group,8 in the continuous exercise group and 8 in the intermittent exercise group.In the diabetes sustained exercise group,there was no interval between continuous exercise for 1h.In the intermittent exercise group of diabetes mellitus,intermittent swimming was performed during the exercise,with 6min of exercise,4min of rest,and 10 groups of alternating exercise.The total exercise time was 1h.The total training time was 6 weeks,6d/w,and the experimental time was from 20:00 to 23:00 every night.When the mice swimming training in 31 ℃ to 33 ℃ temperature control.Results:(1)After a swim for training and intermittent swimming training found: continued swimming training and intermittent swimming training for Ⅱ diabetes mice exist differences in the influence of glucose metabolism index,and comparative analysis by renal biopsy,found that in the renal tubular structure there are significant differences.(2)The last six weeks of swimming training results show: Ⅱ diabetes mice model was established,the weight of DM group of diabetic mice compared with normal control group(Rc),has a significant difference between group(p<0.01),there has been a marked increase weight;The body weight of the mice before the training was significantly different from that of the Rc group,and the blood glucose was increased,reaching the high blood glucose standard.By observing the changes in the state of the mice,the body weight was reduced,indicating that the modeling was successful.After the training,compared with group DE2,the weight of mice in group DE1 was increased in group DG,while the weight of mice in group DE1 was increased significantly(P<0.05).Blood glucose: compared with the DG group,the blood glucose of the DE1 group was significantly decreased after the end of the training at week 6(P<0.05),while the blood glucose of the DE2 group was significantly changed(P<0.01).There was no significant difference between the kidney indexes of group DE1 and group DE2 and the DG group(P>0.05),but there was significant difference between the two groups of mice and the Rc group(P<0.05).Physiological and biochemical indexes:Continuous exercise had a significant effect on triglycerides and blood urea nitrogen indicators,and the effect was extremely significant compared with the DG group(P<0.01).It had a significant effect on the regulation of total cholesterol and glycosylated serum protein(P<0.05),while the effect on creatinine content was reduced,but there was no significant difference(P>0.05).And keep on moving for diabetes mice kidney total superoxide dismutase and malondialdehyde content had extremely significant effect(P < 0.01).Intermittent exercise had a significant effect on the kidney weight of mice(P<0.05),but the effect on the kidney index was not statistically significant(P>0.05).Compared with the DG group,the content of glycosylated serum protein,triglyceride and blood urea nitrogen showed great changes,with extremely significant differences(P<0.01),significant differences in the effect on total cholesterol content(P<0.05),and small effects on the regulation of creatinine,with changes in the data,but no significant differences(P>0.05).Intermittent exercise had a significant effect on t-sod activity and MDA content in the kidney of mice(P<0.01).DE2 and DE1 group than mice,cut down the content of blood urea nitrogen in DE2 mice more,has the extremely significant difference(P<0.01),while the triglyceride content according to the influence of a significant difference(P<0.05),but the DE1 group compared with DE2 team,keep on moving for the influences of total cholesterol(P<0.05),compared to other aspects index had no significant difference(P>0.05).Conclusion:(1)The study found that swimming exercise and intermittent swimming exercise training can effectively improve Ⅱ sugar metabolic disorders in diabetes mice,to ease the deterioration.(2)Continuous swimming exercise was better than intermittent swimming exercise in reducing total cholesterol,but intermittent swimming exercise was better than continuous swimming exercise in regulating blood urea nitrogen,triglyceride and kidney protection in mice.(3)Compared with the changes in renal tissue morphology and structure and degree of fibrosis,the analysis showed that intermittent swimming and continuous swimming both played a certain role in protecting the kidney.Compared with group DE1,the extracellular matrix of renal tubules decreased in group DE2,and the changes in renal fibrosis were more obvious.Thus,60 min swim for effects on diabetes mice falls below 60 min of intermittent movement,so the effects of intermittent movement on Ⅱ diabetes effect is good,have good effect for relief. |
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