Simvastatin Ameliorates Transplant Arteriosclerosis In Rat Aorta Allograft By Inhibiting The Expression Of TLR4

Obejective: To explore the pathological role of TLR4 in Rat allograft vascular lesions and the effects of Simvastatin on it.Methods: The abdominal aorta transplantation between Wistar and SD rats was used as the animal model to observe the development of the transplant arteriosclerosis.The experiment was divided into the isogenic group(group A),allogeneic Simvastatin group(group B),and allogeneic control group(group C).At 70 days,the grafted arteries were harvested,the thickness of the grafted intima was measured,and IHC,HE staining was performed to observe the expression of TLR4,NF-k B,VCAM,IVAM and PCM-1 in each vascular tissue of the grafted blood vessel tissue,and the integrated optical density value of each group was calculated.Results: The intima of the transplanted artery thickened at 70 days after surgery and the highest in group C.Compared with groups A and B,the differences in intima thickness and integrated optical density were significant(P <0.05).The expression of TLR4,NFk B,VCAM,IVAM,and PCM-1,was also the strongest in group C,and the expressions of TLR4,NF-k B,VCAM,IVAM,and PCM-1 in group B were significantly weaker than that in group C(p <0.05).Conclusion:1.The improved vascular cannulation method was used to establish the animal model of thoracic aortic peritoneal transplantation in rats,which can better simulate the occurrence and development of cardiac graft vascular disease.2.The increased expressions of TLR4,NF-k B,VCAM,IVAM,and PCM-1,may be involved in the development of vascular graft disease and is highly correlated.3.Simvastatin ameliorates the progression of vascular graft disease may by inhibiting the expression of TLR4,NF-k B,VCAM,ICAM,and PCM-1.