Polymorphism Of HCMV Glycoprotein Among Infected Patients And Its Induction On TSDF Apoptosis

Human cytomegalovirus(HCMV)is prevalent in worldwide,which seriously threat the health of people with low immunity and newborn babies.Because of its universality,it is often not paid enough attention in clinical practice.In current study,samples of patients with HIV/AIDS complicated with HCMV infection,patients with COPD complicated with HCMV infection and patients with abortion were collected respectively for further study.Genes of HCMV g B(UL55),g N(UL73)and g O(UL74)were amplified and subsequently sequenced.Furthermore,the polymorphism of these three genes were analyzed to explore the influence of HCMV genotypes on disease progression and related clinical indicators.The samples of HCMV virus nucleic acid were extracted,and the viral load was determined by real-time fluorescence quantitative PCR,and the glycoprotein gene subtypes were identified by multiple nested PCR and RFLP(restriction fragment length polymorphism).Moreover,nucleotide sequences were sequenced,and establish an evolutionary tree to analyze their homologous relationship,so as to understand the variation of nucleotide sequences in high-variation regions.We found that patients with mixed infection of multiple HCMV genotypes had lower CD4+ T lymphocyte Numbers,and mixed infection of multiple HCMV genotypes was likely to impair immunity of AIDS patients.Compared with common abortion patients,fetal abortion patients with HCMV detection rate is higher,and g N4 genotype associated with congenital infection detection rate is higher.COPD patients with HCMV multiplex infection rate is the highest of the three diseases.This indicates that multiple mixed infection of HCMV is not uncommon in clinical practice and has adverse effects on the course of disease.The gene of envelope glycoprotein is polymorphic,and the variation is very obvious in the hypervariable region,so there are many subtypes.However,no influence of certain subtypes on virulence was found,and no geographical and regional differences were observed.In vitro study of HCMV,on the basis of cross-species infection of TSDF cells by HCMV,apoptosis inhibition experiments were carried out on cell apoptosis induced by virus to explore the effect of apoptosis on replication of HCMV virus.The results showed that the species-specific HCMV was difficult to replicate and proliferate in cells of other species,and the apoptosis caused by the virus may be only a small part of this complex mechanism.In conclusion,the clinical infection of HCMV needs to be paid attention to,especially in patients infected with multiple strains.Because the multiple infection of HCMV will aggravate the disease and make the patient’s life and health more threatened.In addition,TSDF apoptosis induced by HCMV cross-species infection cells can be inhibited to some extent,but inhibition of cell apoptosis alone does not seem to help the replication and proliferation of the virus,which requires further exploration of the species-specific mechanism.