The Association Between HBV Genotypes And HBV Relative Liver Diseases And Immune Status

Background:Hepatitis B virus is widely prevalent in China. The HBV genotypes are characterized by geographic distribution, as well as associated with clinic outcome and prognosis.Aim:To investigate the distribution and features of HBV genotypes in GuangXi province, as well as the association between the HBV genotype and the clinical manifestation. The association between the HBV genotype and T cell immune response was also studied.Methods:Serum samples were collected from asymptomatic carrier (ASC), liver cirrhosis (LC), chronic hepatitis B (CHB) and hepatocellular carcinoma (HCC) individuals. Liver function, HBV biochemical markers, HBVDNA and T-lymphocyte subpopulations were measured. Type-specific primers nested polymerase chain reaction was applied for HBV genotyping. The data was analyzed with SPSS17.0. Results:Genotype C was found to be predominant (66.3%), followed by genotype B (33.7%) in this study. HBeAg positive rate of C genotype was significantly higher than B genotype, while the proportion of C genotype in HCC group exhibited significantly higher than the ASC, CHB and LC groups. No significant difference was found in age, viral loads, ALT, AST and T cell subpopulations between B, C genotypes.Conclusions:There were mainly B and C genotypes prevalent in this study, with C type being significantly dominant. HBV genotypes were not associated with the clinical manifestation of liver diseases. However, C type was associated with HCC. CD8(+)T played a key role in the viral clearance and liver damage. The study found no association between HBV genotypes and the cellular immunity, which suggests a potential field to be explored.