Characterization Of Reproductive Diapause And Biological Control In Spotted Wing Drosophila,Drosophila Suzukii

Drosophila suzukii native to Southeast Asia,has strong reproductive and survival ability which damage to a verity of fruits.In the past few decades,it has expanded its infection range,affecting major fruit producing areas around the world,causing great economic losses.Since the current control measures rely heavily on pesticides,which have exceeded the maximum residue level and affected fruit output,the use of integrated pest management strategies can significantly reduce the input of pesticides.To study the current pest control measures of D.suzukii,the genetic and biological control measures were explored through researching the effects of insulin signaling on reproductive diapause and high temperature on Trichopria drosophilaeThe protein antibodies were prepared for this study.Recombinant expression vectors of YP1 and Fox O of D.suzukii were constructed.The recombinant proteins were expressed and immunized mice to prepare antibodies.The results showed that the fusion proteins were expressed in Escherichi.coli.The polyclonal antibody had great specificity and the band was still clear after diluted 18000 times.The results indicate that Ds-YP1 and Ds-Foxo polyclonal antibodies could be used in subsequent study.The regulatory factors related to reproductive diapause in D.suzukii were explored through the following ways.Compared with the non-diapause group,the diapause group had smaller ovary,lower expression of YP1 protein,lower insulin titer,higher triglyceride content,significantly larger lipid droplets,lower transcription level and phosphorylated protein expression of ⅡS key genes In R,Akt and Fox O in D.suzukii.These results suggest that insulin signal may be involved in regulation of reproductive diapause.In order to determine whether insulin signal is involved in regulating reproductive diapause,2 hours old adult females were injected with bovine insulin under diapause conditionsor.The group of HNMPA-（AM）3 was bred in non-diapause conditionsor.PBS was injected as control group.After injection bovine insulin 10 and 15 days,ovaries were larger,YP1 protein expression was higher,insulin titer was higher,TG content was lower,transcription levels of Ds In R,Ds Akt,Ds Fox O and phosphorylated protein expression were higher.The changes of ovarian development,YP1 protein,TG content,transcription levels and phosphorylated protein levels of HNMPA-（AM）3 injection were detected synchronously.The results were opposite to group of bovine insulin injection.These results indicate that insulin signal can regulate D.suzukii reproductive diapause through ⅡS.The effect of high temperature on T.drosophilae was investigated to provide practical basis for field release of T.drosophilae.It was found that the prepupal stage was more resistant to high temperature stress than the larval stage by studying the emergence rate,parasitism rate,developmental duration and dead pupal rate.The wasps could survive and emerge successfully when prepupal stage at 30℃,develop at 35℃ but could not emerge,and could not develop at 40℃.The larval stage at 30℃ within 24 h,the wasps could survive and emerge successfully,but wasps were nearly dead at 30℃ more than 24 h.The wasps could not develop at 35℃ and 40℃.Therefore,when releasing parasitic pupae in the field,it is recommended that the temperature in the shed should not exceed 30℃,and the later the development stage,the better the temperature tolerance.