Effect Of Hydrogen On The Preservation Effects Of Boar Semen

Semen preserved under normal and ultra-low temperature condition is the main product of boar stations.The quality of semen directly determines the effects of artificial insemination and affects the economic profits of swine production enterprises.Therefore,how to enhance the preserving effects of boar semen has been a hot topic in the field of porcine reproduction.Studies showed that respiration metabolism of sperm produces plenty of ROS,leading to the damage of sperm membrane and causing a sharp reduction in the fertilized competence of sperms.Hydrogen has been proved to inhibit oxidized damage of the cells.Thus,we explores in this study the effects of hydrogenon semen stored under normal ultra-low temperature condition.These studies will provide a theoretical support in improving the preserving techniques of boar semen.The results are listed below:The effects of hydrogen on the quality of boar semen preserved under normal temperature.Diluent added with hydrogen served as the experimental group(S group),and diluent without hydrogen served as the control group(D group).Compared to D group,T-AOC of sperms in S group on day0(84.27±6.82 Vs 44.02±7.34,P<0.05),day 2(81.45±4.76 Vs 43.80±6.20,P<0.05),day 4(80.96±5.66 Vs 43.50±4.07,P<0.05),day 12(74.82±10.47 Vs 40.06±5.69,P<0.05)significantly increased.For survival rates of sperms,compared to D group,survival rates of sperms in S group on day 8(88.41±1.38 Vs 83.97±2.2,P<0.05),day10,(89.68±1.07 Vs 87.69±1.00,P<0.05),day12,(90.72±1.72 Vs 86.4±1.56,P<0.05),day14,(88.47±1.12 Vs84.15±1.16,P<0.05)apparantlyincreased.For mitochondrial function of sperms,compared to D group,mitochondrial activity of sperms in S group on day 6(95.44±1.07 Vs 91.43±1.21,P<0.05),day8(85.89±0.90 Vs 83.27±0.76,P<0.05),day12(85.51±0.45 Vs 82.37±1.03,P<0.05)significantly enhanced.The effects of hydrogen on the quality of boar semen preserved under ultra-low temperature condition..Hydrogen was added to pre-diluent,cryopretective diluent Ⅰ,and cryopretective diluent Ⅱ,respectively.“+” represents the addition of hydrogen,“-”indicates the absence of hydrogen.Three diluent without the addition of hydrogen served as the control group(---).Results showed that MDA of frozen-thawed sperms in(+--)group,(+-+),(+-+)group(302.21±17.69 Vs 256.92±4.25,P<0.05),(++-)(302.21±17.69 VS 211.94±10.60,P<0.05),(+++)group(302.21±17.69 Vs 187.10±20.93,P<0.0)significantly decreased compared to the control group.For T-AOC,T-AOC of frozen-thawed sperms in(++-)group(142.78±6.73 Vs114.44±12.95,P<0.05),(+-+)group(169.45±17.51 Vs 114.44±12.95,P<0.05),(+++)group(208.33±11.67 Vs 114.44±12.95,P<0.05)obviously increased.For motility of sperms,compared to the control group,survival rates of frozen-thawed sperms in(++-)group(67.80±7.55 Vs 48.04±5.52,P<0.0),(+++)group(68.96±5.28 Vs 48.04±5.52,P<0.05)significantly increased.For viability of sperms,compared with the control group,(-+-)grou(44.22±1.27 Vs 35.72±1.26,P<0.05),(-++)group(46.58±3.00 Vs 35.72±1.26,P<0.05),(+-+)group(44.06±4.22 Vs 35.72±1.26,P<0.05),(++)group(49.62±3.71 Vs 35.72±1.26,P<0.05),(+++)group(50.00± 3.67 avs 35.72±1.26,P<0.05)sperm viability was significantly increased in both freezing and thawing.For mitochondrial function of sperms,compared to the control group,mitochondrial activity of frozen-thawed sperms in(+-+)group(85.87±1.43 Vs 68.67±8.9,P<0.05),(++-)group(86.2±1.83 Vs 68.67±8.9,P<0.05),(+++)group(87.83±7.00 Vs 68.67±8.9,P<0.05)significantly increased.For the percentage of spermswith acrosome integrity,compared to the control group,percentage of frozen-thawed sperms with acrosome integrity in(+--)group(79.14±2.18 Vs 62.09±7.95,P<0.05),(+-+)group(79.14±2.18 Vs 62.09±7.95,P<0.05),(++-)group(79.14±2.18 Vs 62.09±7.95,P<0.05),(+++)group(79.14±2.18 Vs 62.09±7.95,P<0.05)obviously elevated.In conclusion,the antioxidant,MDA index,activity,plasma membrane integrity and mitochondrial activity of semen had positive effects on the preservation of fresh semen diluent.Compared with the control group(--),the experimental group adding hydrogen in the pre-diluent had better effects,and it had positive effects on MDA indexes,antioxidant indexes,vitality,activity rate,plasma membrane integrity rate and mitochondrial function.These results can provide technical support for the optimization of normal temperature and cryopreservation conditions of pig semen and the application and popularization of frozen semen in pig industry.