| Trichomoniasis of pigeons is a serious spread parasitic disease.It often occurs in squabs aged 2～5 weeks.Serious infection will lead to death and seriously hinder the development of pigeon breeding industry.In production,the disease is mainly prevented and treated by regular use of antibiotics and traditional Chinese medicine extracts.Long time use of drugs to control,there are drug-resistant insect strains.Probiotics have become recognized as safe and effective green additives and have been applied in livestock and poultry breeding.It is expected to become a new way to control T.gallinae of dove.T.gallinae used in this study was isolated from the saliva of seriously ill pigeons,purified,cultured and identified for follow-up tests.Firstly,five strains of pigeon derived Lactobacillus were selected for in vitro screening,and the Lactobacillus with the best anti insect effect were selected through the insect inhibition rate to carry out animal experiments to explore the effect of Lactobacillus on anti T.gallinae infection of suabs.Select 100 squabs of similar weight and7-day-old white King pigeon without T.gallinae infection,they were randomly divided into 5 groups:A(negative control group),B(positive control group),C(metronidazole treatment group),D(Lactobacillus prevention group)and E(Lactobacillus treatment group),each group of 20 squabs were raised in a strictly disinfected environment without T.gallinae.During the age of 7～14days,group D was fed with Lactobacillus 2×10~8cfu/mL on an empty stomach before feeding every day,and the other four groups were fed with MRS culture medium of 2ml each.At the age of 14 days,except group A,each pigeon in other groups was infected with T.gallinae by mouth 1×10~7.After squabs infection,Group A and group B were fed with 2 ml MRS culture medium on an empty stomach before daily feeding,Group D and group E each received 2ml of 2×10~8cfu/mL Lactobacillus.At the age of 21 days,8pigeons in each group were randomly selected for sampling,and the collected samples were tested accordingly.The specific test results are as follows:(1)A strain with 99.69%comparison rate with ITS5.8s gene of T.gallinae pigeon was isolated and identified as T.gallinae,which can be used in subsequent experiments;(2)After squabs were infected with T.gallinae,the average daily gain of the group B decreased significantly(P<0.05),and the average daily gain of group C,group D and group E was not significantly affected;The number of oral T.gallinae in group C and group D was significantly lower than in group B(P<0.05);Compared with group A,the group B white blood cell count and monocyte decreased significantly,there was no significant difference among group C,group D and group E(P>0.05).(3)The 16S diversity analysis of crop contents showed that compared with the positive control group,the abundance of Lactobacillus increased and the abundance of harmful Gallibacterium decreased in the Lactobacillus prevention and cure group;(4)It was analyzed by eukaryotic transcriptome sequencing technology and found that,under the stimulation of T.gallinae infection,the bactericidal permeability enhancing protein in the metronidazole treatment group was significantly expressed,and the intestinal immune network signal pathway producing IgA was enriched.The administration of lactic acid bacteria promoted the significant expression of Toll like receptor 7 and MYD88 immune related genes in squabs,these pathways may play a key role in the resistance of squabs to T.gallinae infection,improve the immunity of pigeons,improves the immune response of the body,and promotes the body to quickly remove pathogens. |
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