Screening And Preliminary Function Analysis Of Wheat OFPs,MBF1s And BSDs Genes Related To Drought And Heat Stress

Wheat was an important food crop in the world.Different stresses such as high temperature and drought seriously affect wheat growth and yield.The transcription factors OFPs,MBF1 s and BSDs were involved in plants growth and stress response unreported in wheat.In this study,Comprehensive analysis of OFPs,MBF1 s and BSDs Using Bioinformatics in wheat.Screening out genes related to drought and heat stress in wheat OFPs,MBF1 s and BSDs.And exploring the expression and preliminary function of candidate gene resources for the breeding of new wheat varieties.The main results are as follows:1.In wheat,100 OFPs genes were identified through a genome-wide survey and classified into four subfamilies;Gene structure analysis revealed that only 4 OFPs genes had introns;Gene duplication analysis revealed that Ta OFPs had three tandem duplicated genes and one pairs of duplicated gene segments;Promoter analysis suggested that most Ta OFPs had abiotic stress elements,Among them,53 genes contain MBS cis-acting elements that were related to drought stress;Tissue-specific analyses showed that Ta OFP17-A.1/A.2,Ta OFP17-B.1/B,Ta OFP19,Ta OFP29a-A and Ta OFP3-B were specifically expressed in roots,Ta OFP21-A and Ta OFP22-D was specifically expressed in leaves,Ta OFP24-A.2,Ta OFP24-A.3 and Ta OFP25-D were specifically expressed in seeds;Further analysis of gene expression patterns under abiotic stress revealed that Ta OFPs were expressed in varying degrees of drought and heat stress.Among them,Ta OFP3-B,Ta OFP9-D,Ta OFP12-D and Ta OFP29a-A were up-regulated by drought stress,Ta OFP17-B.1/B.2 and Ta OFP17-A.1/A.2 were up-regulated by heat stress.Overexpression of Ta OFP29a-A could improve the drought tolerance in wheat.2.In wheat,nine MBF1 s genes were identified through a genome-wide survey and classified into two subfamilies;Gene structure analysis that only 4 OFPs had introns;Gene structure analysis revealed that most members contained multiple exons,of which Ta MBF1 c contained one exon;Promoter analysis suggested that most Ta MBF1 s had MBS and HSE that were related to abiotic stress,among them,Ta MBF1b-A and Ta MBF1c-D were 5 MBS that were related to heat stress;Tissue-specific analyses showed that Ta MBF1c-A/B and Ta MBF1 a were expressed in leaves;Further analysis of gene expression patterns under abiotic stress revealed that Ta MBF1 s were induced by drought and heat in different degrees expression.Compared with drought-sensitive varieties,Ta MBF1 a was highly expressed in drought-tolerant varieties,while the expression levels of Ta MBF1 b and Ta MBF1 c decreased significantly in drought-tolerant varieties by drought stress.Compared with heat-sensitive varieties,Ta MBF1 a,Ta MBF1 b and Ta MBF1 c genes were largely induced to expressed in heat-tolerant varieties by heat stress.3.In wheat,33 BSDs genes were identified through a genome-wide survey and classified into three subfamilies;Gene structure analysis revealed that most members contained multiple exons,Among them,Ta BSD2,Ta BSD4 had only one exon;Promoter analysis suggested that most Ta BSDs had abiotic stress elements;Tissue-specific analyses showed that Ta BSDs might serve tissue-specific,Among them,Ta BSD1,Ta BSD4-D,Ta BSD5-D,Ta BSD6 b and Ta BSD8-B were specific in roots Expression,Ta BSD3-A was specifically expressed in leaves,and Ta BSD10-B was specifically expressed in seeds;Further analysis of gene expression patterns under abiotic stress revealed that Ta BSDs can be expressed in varying degrees of drought and heat induction.Among them,Ta BSD1-A,Ta BSD2-A/D,Ta BSD3-D,Ta BSD5-D,Ta BSD7-A,Ta BSD8-B and Ta BSD9-A showed up-regulated expression by drought stress,Ta BSD5-B/D,Ta BSD7-A,Ta BSD8-A/D,Ta BSD9-A,Ta BSD10-A/D and Ta BSD6a-B showed up-regulated expression by heat stress.