Wide-scope Multi-residue Analysis Of Pesticides In Beef By High-Resolution Mass Spectrometry

Increasing pesticide contamination in foods of animal origin has made the widescope multi-residue analysis of pesticides an international concern.Therefore,it is important to establish methods for the determination of multiple pesticides in beef substrates.In this essay,there are two quantification methods based on HRMS have been developed.Firstly,a sensitive and reliable multi-residue pesticide analysis method for beef was developed with the use of 129 pesticides to demonstrate the full scope of the method.The pesticides were extracted and purified using a modified QuEChERS technique and analysed using ultra-high-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry.An orthogonal array design was employed for optimisation of the method,significantly scientising and simplifying the process.Method validation revealed satisfactory sensitivity with limits of quantification values ranging from 0.003 to 11.37 μg/kg.Matrix effects ranged from 83.85%to 120.66%.Recovery at three spiking levels(20,50,and 100 μg/kg)were 70.51-28.12%and the intra-day and inter-day precisions were less than 20%,indicating good accuracy and precision.Secondly,another sensitive and reliable multi-residue pesticide analysis method for beef was developed with the use of 197 pesticides to demonstrate the full scope of the method.The pesticides were extracted and purified using a modified QuEChERS technique and analysed using gas chromatography coupled to Orbitrap mass spectrometry.Method validation revealed satisfactory sensitivity with Screening Detection Limit values ranging from 0.1 to 50 μg/kg.Recovery at three spiking levels(100,200,and 500 μg/kg)were 73.04%-113.97%and the intra-day and inter-day precisions were 0.27%-18.29%,indicating good accuracy and precision.