Effects Of Feeding Different Levels Of Soy Isoflavones On Dairy Cow Performance And Serum Biochemical Indicators

In order to explore the effects of different levels of SIF on milk production,immune performance,serum biochemical indicators and fat metabolism in dairy cows.Forty cows of Chinese Holstein were selected for the experiment and randomly divided into 4 groups according to milk production,lactation days and parity pairings:control group,experimental group I,experimental group Ⅱ and experimental group Ⅲ.The control group was fed a basal diet,and the experimental group was fed a SIF of 5,15 and 30 mg/kg in addition to the basal diet The preliminary experimental period is 7 days,and the positive experimental period is 60 days.The experimental is divided into two phases,each phase is 30 days.The results of experiment 1 showed that during the first 1 to 60 days of the experiment,the average daily milk production of the experimental group Ⅲ increased by 20.77%compared with the control group,a significant difference(P<0.01).Compared with the control group,the amount was increased by 13.60%and 11.34%(P<0.01);the milk protein content of the experimental group Ⅲ was significantly higher than that in the control group,the experimental group Ⅰ and the experimental group Ⅱ(P<0.01);The number of somatic cells was significantly higher than that in the experimental group(P<0.01).The results showed that the milk yield and milk protein rate of the cows were improved,and the increase in the number of somatic cells was suppressed because of the 30 mg/kg SIF.The results of experimental 2 showed that on the 60th day of the experimental,the activity of CAT in the cow serum of the experimental group Ⅱ and group Ⅲ was significantly higher than the control group(P<0.05).On the 30th day of the experimental,the activity of T-AOC in the serum of cows in the experimental group Ⅲ was significantly higher than that in the control group(P<0.01).On the 30th day of the experimental,the content of Ig A and Ig G in the serum of the cows in the experimental group Ⅲ were significantly higher than those in the control group(P<0.01).On the 60th day of the experimental,the content of Ig G in the serum of the experimental group Ⅲ and experimental group Ⅱ All of them were significantly higher than in the control group(P<0.01).From day 1 to day 60 of the experiment,the content of IFN-γ in the serum of the cows in the experimental group Ⅲ was significantly higher than that in the control group(P<0.01).This showed that the improvement of the immune performance and antioxidant capacity of the cows is due to the addition of 30 mg/kg SIF.The results of the 3 experimental showed that at the 30th day of the experimental,the AST activity of the experimental group Ⅲ was significantly lower than that in the control group(P<0.01),and the AST activity of the experimental group Ⅰ and experimental group Ⅱ was significantly higher than that in the experimental group Ⅲ(P<0.05).On the 60th day of the experimental,the AST activity of the experimental group Ⅲ was significantly lower than that in the control group(P<0.01),and the AST activity of the experimental group Ⅰ and experimental group ⅡI was significantly higher than that in the experimental group Ⅲ(P<0.05);compared with the TP content of the group,the difference was extremely significant(P<0.01);the TP content of the experimental group Ⅰ was significantly different from that in the control group(P<0.05).The TP content of dairy cows is increased,the activity of AST is reduced,and the internal environment is improved,and lactation performance is improved because of the addition of 30 mg/kg of soybean isoflavones.The results of experimental 4 showed that at the 30th day of the experimental,the TGH activity of the experimental group Ⅱ and the control group was significantly lower than that in the experimental groupⅢ and the experimental group Ⅰ(P<0.01);the control group FASN was significantly lower than that in the experimental group Ⅰ and experimental group Ⅲ(P<0.05).On the 60th day of the experimental,the TGH activity of the control group was significantly lower than that of the experimental group Ⅱ and the experimental group Ⅲ(P<0.05);the NEFA content of the experimental group Ⅱ and the control group was significantly higher than that in the experimental group Ⅲ(P<0.01).At the 0-60th day of the experimental,the FASN of the experimental group Ⅲ and the experimental group Ⅰ was significantly higher than that in the control group(P<0.05);the GGT of experimental group Ⅲ was significantly higher than that in the control group(P<0.01)and significantly higher than the experimental group(P<0.05);the TGH activity of the control group was significantly lower than those of the experimental group Ⅱ and the experimental group Ⅲ(P<0.01),and the experimental group Ⅰ was significantly higher than that in the experimental group Ⅱ(P<0.05);Higher than the control group(P<0.01),significantly higher than the experimental group Ⅰ and Ⅱ.(P<0.05);the content of TG in the control group was significantly higher than that in the experimental group Ⅰ(P<0.01),and significantly higher than the experimental group ⅢAnd experimental Ⅱ group(P<0.05).This showed that the fat metabolism of dairy cows is improved because of the addition of soy isoflavones,and the best effect is when 30 mg/kg SIF is addedIn summary,in this experiment,due to the addition of 30mg/kg of SIF,the production performance of dairy cows has been improved,the fat metabolism and energy metabolism of dairy cows have been promoted,and the antioxidant and immune performance of dairy cows have also been improved.