Study On The Mechanism And Effect Of Resveratrol Against Koi Herpes Virus

Koi Herpes virus of carp,highly contagious,is one of the major diseases which will cause massive death once infected.Death rates are as high as 80%-100% and will bring huge losses to the crap industries.There are no specific drugs for the disease presently but the widely use of broad-spectrum antibiotics has caused the pollution of water and environment.However,the herbal medicine has natural substances,no residue in the body as well as no pollution of water and environment.Therefore,it is of great significance to explore Chinese herbs with natural anti-inflammatory,anti-oxidation and anti-virus effects.Antioxidant,anti-inflammatory and antiviral effects of Resveratrol have been confirmed by domestic and foreign scholars in recent years.The purpose of this experiment is to explore the mechanism and effect of resveratrol on Koi herpes virus(KHV),and to screen out the best concentration of resveratrol.In this paper,300(25±5)g koi were used as experimental animals,and different concentrations of 20 mg/kg,40 mg/kg,80 mg/kg resveratrol and 0 mg/kg of the control group were injected intraperitoneally.72 hours after injection,liver and pancreas,kidney,gill,and serum tissues were collected from different concentrations.Take liver and pancreas and kidney tissues to determine the antioxidant genes Maf(Minor Allele Frequency),Nrf2,Keap1,HO-1(recombinant human heme oxygenase1),GPX(glutathione peroxidase),SOD(The relative expression of superoxide dismutase)m RNA,SOD and CAT,the enzyme expression activity of glutathione peroxidase(GSH-PX)and MDA(malondialdehyde)and T-AOC(total antioxidant capacity)and NO(nitric oxide)antioxidants,as well as the relative expression of anti-inflammatory genes I-κB,p65,NF-κB,IN-OS m RNA and IL-8(interleukin-8),IL-10(interleukin-10),TNF-α(tumor necrosis factor-α)inflammatory factor content.The results showed that it was detected by q PCR technology that intraperitoneal injection of resveratrol could increase the expression of Maf,Nrf2,HO-1,GPX,SOD in liver,pancreas and kidney(P<0.05),and decrease the expression of inhibitory protein Keap1 m RNA.(P<0.05).Resveratrol can significantly increase the activity of SOD,CAT,GSH-PX and other antioxidant enzymes in the liver and pancreas,kidneys,gills and serum and the level of T-AOC(P<0.05),but reduce the content of MDA and NO(P <0.05).It was detected by q PCR technology that intraperitoneal injection of resveratrol could increase the expression of I-κB and IL-10 m RNA in liver,pancreas and kidney(P<0.05),and decrease TNF-α,p65,NF-κB,IL-10,Relative expression of IN-OS m RNA(P<0.05).When the concentration of resveratrol is 20 mg/kg,the effect of expression is the best.Intraperitoneal injection of Koi herpes virus 10 times titer(TCLD50titer=10-6.75)was used to preserve the cell virus liquid in our laboratory.After 7days of challenge,samples were taken to determine the relative expression of antioxidant gene m RNA in liver and pancreas and kidney tissues.The relative expression of anti-inflammatory gene m RNA was measured,and the antioxidant substances in different tissues of liver and pancreas,kidney,gill and serum were measured,and histopathological section observation was performed.The results showed that After Rsv injection,the expressions of Maf,Nrf2,HO-1,GPX,SOD,I-κB,and IL-10 in the liver,pancreas and kidney were significantly higher than those in the challenge group(P<0.05),Keap1,TNF-α,The expression levels of p65,NF-κB,IL-10,and IN-OS m RNA were significantly lower than those in the challenge group(P<0.05).After injection of Rsv,the activities of SOD,CAT and other antioxidant enzymes in liver and pancreas,kidneys,gills and serum,as well as the levels of GSH-PX and T-AOC were significantly higher than those of the challenge group(P<0.05),and the levels of MDA and NO Significantly lower than the challenge group(P<0.05).Above that,Rsv can enhance the body’s ability to resist oxidation by activating the message channel of Nrf2-Keap1/ARE.It also can control NF-κB message channel to enhance the body’s anti-inflammatory action.And it has the best antioxidant and anti-inflammatory effects when the resveratrol concentration is 20 mg/kg.The anti-oxidation and anti-inflammatory effects were the best,the protective rate was87.5 %,and the therapeutic effect was the best.