Study On The Prevention And Control Effects Of Diallyl Trisulfide(DATS) On Main Diseases Of Solanaceae Plants

Potato(Solanum tuberosum L)and tomato(Lycopersicon esculentum Mill)are two important solanaceous crops,rich in a variety of nutrients and are important vitamin sources for humans.Crop security and food security are deeply affected by plant diseases.Potato late blight caused by Phytophthora infestans and tomato gray mold caused by Botrytis cinerea infection are the main plants that restrict the development of these two major Solanaceous crop industries Disease.Biological control is one of the alternatives to traditional chemical control and an important research direction for plant disease control.This research uses DATS(Diallyl trisulfide,a sulfide from garlic)to explore the inhibitory effect of Phytophthora infestans and Botrytis cinerea.In addition,through the analysis of the regulation of the disease resistance defense network of potato tubers.All results of this study are as follows:(1)The inhibitory mechanism experiment of DATS on Phytophthora infestans and Botrytis cinerea: DATS(100 μg/L)inhibited the hypha growth and spore germination of Phytophthora infestans and Botrytis cinerea.DATS inhibited the biological viability and membrane integrity of the spores of Botrytis cinerea.and reduces the infectivity of Phytophthora infestans and Botrytis cinerea on potato and tomato.(2)Physiological and biochemical effects of DATS on potato tubers infected by Phytophthora infestans: Fumigation of potato tubers with Phytophthora infestans was conducted in a confined space with 100 ug/LDATS.The 3rd,5th,and 8th day-long experimental potato tuber tissues were taken as samples for the determination of oxidase activity.The results showed that DATS had a strong influence on the CAT of potato tubers,while other antioxidant enzymes(POD,SOD)and MDA content have no significant effect.(3)DATS regulation analysis of potato tuber disease resistance defense network:100 ug/LDATS was used toinoculate potato tubers with Phytophthora infestans for a 7days experiment with miRNA and m RNA transcriptomics analysis.The results showed that a total of 60 and 607 significantly differentially expressed miRNAs(DEM)and genes(DEG)were detected,respectively.Approximately 15 negatively regulated miRNA-m RNA interaction pairs were observed in the miRNA-m RNA co-expression regulatory network.These pairs mainly focus on metabolic pathways,the biosynthesis of secondary metabolites,and starch and sucrose metabolism based on the KEGG pathway.Importantly,DATS stimulates reactive ethylene and oxygen(ROS)signals and reduces jasmonic acid signals,thereby enhancing resistance to potato late blight.(4)Hysiological and biochemical effects of DATS on tomato infected by Botrytis cinerea: DATS significantly affects the antioxidant enzyme activities such as POD and CAT of tomato,By influencing the level of antioxidant enzyme activity in tomato,DATS regulates the defense strength of tomato against Botrytis cinerea.