Difference Study On Fuding Dabai Tea Leaves At Different Developmental Stages Based On Transcriptome Analysis

Tea is an important economical plant.The new shoots of tea can be processed into different types of tea with unique flavors after different types of processing.At present,tea has become the No.1 non-alcoholic beverages in the world.It has great economic,health and cultural value.Tea are rich in secondary metabolites such as tea polyphenols,caffeine,and theanine.These secondary metabolites are significantly different in different tissues and different development stages of tea plant.Therefore,this work takes one bud with one leaf,one bud with two leaf,one bud with three leaf,one bud with four leaf,and old leaf based on transcriptome sequencing data in order to explore the secondary metabolites during the growth and development process of Fuding Dabai tea.The main results are as follows:(1)Gene expression were be analyzed of Fuding Dabai tea at Different Developmental Stages.The transcriptome analysis tools,Fast QC,hisat2,feature Counts,are used to get the gene expression of five tissues of Fuding Dabai tea.The transcriptome data obtained 417 071 169 sequences.The average Q30 is 93.78% and the average GC content is44.47%.The original reads have been aligned to the Shuchazao reference genome.The average blast rate of each sample is 86.22%.The short reads aligned to the reference genome have been standardized to obtain the FPKM of the samples.The results showes that one bud with one leaf and one bud with four leaf have the hightest gene expression.The lowest gene expression tissue is one bud with three leaf.(2)Differentially gene expression were be analyzed at diffferent tissue of Fuding Dabai tea.The transcriptome analysis tools,feature Counts,DESeq2,are used to establish differentially expressed gene matrix for the five growth stages of Fuding Dabai Tea.The differentially expressed genes between each sample were obtained when we finished the differential gene expression analysis of each sample.The results shows that the largest difference between the sample is one bud with one leaf and old leaf.There are 6758 differential genes between them.The smallest difference between the samples is one bud with one leaf and one bud with two leaf.There are 103 differential genes between them.(3)Differential gene function annotation and enrichment analysis were be analyzed between one bud with one leaf and one bud with four leaf of Fuding Dabai tea.We use gene funciton annotation and enrichment analysis to explore the differential expression of different genes in order to reveal their biological functions.The GO annotation results shows that differential genes are most annotated in molecular functions.The KEGG annotations results shows that differential genes are most annotated in metabolism.The KEGG enrichment analysis are enriched in phenylpropane biosynthesis,flavonoid biosynthesis,glutathione Metabolism and etc.(4)KEGG metabolic pathway were be analyzed between one bud with one leaf and one bud with four leaf of Fuding Dabai tea.We analyzed the phenylpropane metabolic pathway,flavonoid metabolic pathway and glutathione metabolic pathway of Fuding Dabai tea.The results of metabolic pathway shows that there are 15 genes correspond to8 enzymes involved in phenylpropane metabolic pathway.There are 6genes correspond to 4 enzymes to regulate flavonoid metabolic pathway.There are 6 genes correspond to 5 enzymes participate in glutathione metabolic pathway.The discovery of these key enzymes revealed differences about metabolism of phenylpropane,flavonoids and glutathione compounds in tea plants.(5)Protein-protein interaction were be analyzed between one bud with one leaf and one bud with four leaf of Fuding Dabai tea.We use STRING database to constructe protein-protein interaction network.The up-regulated differential protein-protein interaction network contains 720 nodes and 750 edges.The down-regulated differential protein interaction network contains 1140 nodes and 2655 edges.After analyzing the protein-protein interaction network,we discovered that protein interactions are highly aggregated.The highly interactions between these proteins plays an important role in the secondary metabolism and growth process in tea plant.The specific regulation mechanism between them needs further research in the future.The article helps to clarify the differences in gene expression and metabolic pathways of Fuding Dabai tea leaves at different developmental stages.It Could provide a theoretical basis for the regulation of tea plant secondary metabolism network.