Regulatory Role Of TFDP-1 In The Restart Of Diapause Embryos In Artemia Sinica

Artemia sinica has strong adaptability to the environment,when encountering external adverse conditions,it can produce diapause embryo to resist the extreme environment.When the external conditions are suitable for growth again,its embryo can break the diapause and continue to develop.TFDP-1 is an important member of the DP family of transcription factors(Transcription factors).It can combine with transcription factors E2F1～6 to form a heterodimer,which greatly enhances the binding ability of E2 F and DNA,so that the combination can activate the transcription of its downstream target genes and then regulate the cell cycle,and is a key protein for cell regeneration.It is also a key protein in cell regeneration.Relevant studies have shown that TFDP-1 plays an important role in diapause relief of Artemia sinica,but the corresponding mechanism in the process of embryo restart is still unknown.The full length of TFDP-1 gene is 712 bp,the total length of open reading frame(ORF)is420,it can encode amino acid 139 aa,the molecular weight of the protein is 15.7KDa,the theoretical isoelectric point is 7,it is a non transmembrane hydrophilic protein.Subcellular localization predicts that a large number of TFDP-1 are expressed in the nucleus and cytoplasm It is predicted on line that it contains a DP domain.Real time PCR and Western blotting showed that TFDP-1 gene and protein expression were deeply affected by the development period,salinity and temperature of Chinese Artemia.When the environment was not suitable for Artemia growth,TFDP-1 expression level was rapidly down regulated to cope with the coming harm;when the environment was suitable,TFDP-1 expression was raised to a certain level again,so as to control the activation of its downstream target genes The progress of cell cycle.In this paper,we have further research on the key cyclin TFDP-1,and have a deeper understanding of the restart of Artemia diapause embryos,which provides a scientific basis for human beings to uncover the mystery of stress resistance and the mechanism of cell growth and development.