Identification And Expression Analysis Under The Drought And Salt Stress Of 14-3-3 And BES1 Gene Family In Nitraria Tangutorum

The 14-3-3 gene family is involved in many basic links in the cell life process,it plays an important role in plants growth and development,resistance to stress,and signal transduction.Plant-specific transcription factor BES1,it is a key transcription factor in the BR signaling pathway in brassinolide,it also plays an important role in plant growth and development and enhancing plant resistance.In model plants,researchers have conducted extensive studies on the 14-3-3 and BES1 gene family.However,there are few studies on the 14-3-3 and BES1 gene family in Nitraria tangutorum.The work is based on the transcriptome data of N.tangutorum,bioinformatics methods were used to identify the members of the 14-3-3 and BES1 gene family in N.tangutorum,predict its physicochemical property,subcellular localization,conserved domains,phosphorylation sites,and analyze the evolutionary relationship of the system,function notes,and the tissue and organ table of its genes under different concentrations of salt and drought expression pattern.In order to identify N.tangutorum 14-3-3 and BES1 genes related to abiotic stress response.The result is as follow:1.We identified 10 14-3-3 genes and 7 BES1 genes in the range of N.tangutorum transcriptome data.2.10 14-3-3 family members were preliminarily screened from the transcriptome data of N.tangutorum.Among them,9 members with complete protein（coding sequence CDS）had 212 297 amino acids,the isoelectric point was 4.73 ⁶.14,and the molecular weight range of 24.06 33.51 k Da.The N and C terminus varied greatly,and the rest were conserved amino acids.7 members of N.tangutorum BES1 gene family all have complete CDS,had 86 422 amino acids,the isoelectric point belonged to 5.29 10.22,and the molecular weight range belonged to 9.75 ⁴7.53 k Da,3 genes（NtBES1-1,NtBES1-5 and NtBES1-6）contain BES1_N and Glyco_hydro₁4 included domains,the other 4 genes only contain the BES1_N conserved domain.3.According to the phylogenetic relationship,the 14-3-3 and BES1 genes of N.tangutorum were divided into 4 and 3 groups,respectively.The 14-3-3 gene has a significant increase in the number of genes in group I,it implies that the 14-3-3 gene of N.tangutorum is more intense in the process of evolution,there has been a genetic doubling,and in groups I,II,and III,both have N.tangutorum and Arabidopsis thaliana 14-3-3 genes.Among the members of the BES1 gene family,the degree of aggregation among dicots is higher,the BES1 genes of monocots are all clustered and branched individually,it has low homology with the BES1 gene of dicotyledonous plants,it shows that there are big differences in the evolution of the BES1 gene between monocots and dicotyledonous species.And the BES1 gene has multiple replications in different plants.Compared with other species,in groups I,II,and III,some BES1 genes of N.tangutorum and Arabidopsis thaliana BES1 genes have high homology.The above analysis shows that N.tangutorum 14-3-3 and BES1 gene family are closely related to the Arabidopsis thaliana 14-3-3 and BES1 family,and genes with higher homology may have similar functions.4.KEGG analysis showed that 10 N.tangutorum 14-3-3 genes and 7 BES1 genes were in “MAPK signaling pathway-plant” and “Plant hormone signal transduction” pathways,respectively.It suggests that they may be involved in hormone-mediated growth and development of N.tangutorum or in response to adversity stress.5.Using qRT-PCR technology to analyze the expression of N.tangutorum14-3-3 and BES1 genes under different concentrations of PEG and salt stress,the results show that under different concentrations of PEG and Na Cl treatment,most genes of N.tangutorum 14-3-3 and BES1 were significantly induced,for example,the expression levels of GRF3like-3,GRF2 like and NtBES1-4 all present an upward-regulated trend,it shows that these genes play an important role in N.tangutorum in response to drought and salt stress.6.The study found that under different concentrations of PEG and NaCl treatment,among the 14-3-3 genes,under the control and PEG treatment,GRF3like-3had the highest expression in leaves,under the control,PEG treatment and Na Cl treatment,both GRF3 and GRF2 like had the highest expression levels in roots.In addition to NtBES1-6,3 genes in BES1 gene,under the control and PEG treatment,NtBES1-2 and NtBES1-4 had the highest expression levels in roots,and under the Na Cl treatment,all 3 genes are highly expressed in leaves.The above results show that,N.tangutorum 14-3-3 and BES1 genes show diverse expression patterns in different tissues and organs,and can respond to external drought and salt stress.