High-density Heterotrophic Culture Of Isochrysis Galbana Parke 3011

In this paper,the bait microalgae Isochrysis sphaerocephala 3011 was taken as the research object,and it was cultured heterotrophically in the dark,to study its heterotrophic culture conditions and media,and the preservation conditions of Isochrysis sphaeroides after heterotrophic.Recovery conditions after preservation of Isochrysis.Provide a reference for the future high-density heterotrophic fermentation of microalgae.In the case of Isochrysis heterotrophicus,the types and concentrations of heterotrophic carbon sources,nitrogen sources and phosphorus sources were screened through single factor experiments,and the optimal medium composition was determined through orthogonal experiments:40 g/L glucose,the nitrogen source is 0.6 g/L urea,and the phosphorus source is 1.5 g/L potassium dihydrogen phosphate.Trace elements,hydrating cream and activator have no obvious effect on Isochrysis heterotrophic.The optimal heterotrophic temperature is 23℃.The heterotrophic culture of Isochrysis gallbladder was stored in glucose,sucrose and glycerol solutions.Adding glucose to the concentrated algae solution may be beneficial to the preservation of Isochrysis heterotrophic.The concentrated algae solution was stored in a tightly sealed solution at room temperature for 7 days in a 30 g/L glucose solution with a mass-to-volume ratio of 1:1,and the cell survival rate remained 72.3%.Determine the best recovery conditions for Isochrysis heterotrophic ball preserved for 7 days.Through single factor experiment,the light intensity,aeration volume,inoculation amount,fat and water cream and activator are screened,and the heterotrophic ball Isochrysis is obtained in light.With an intensity of 4000 lx,an inoculum volume of 2.5%,a ventilation volume of 1 L/min,and the addition of fertilizer cream and activin,it can reach 1.8×10~7cells/m L on the 12 th day.Compared with autotrophic culture and heterotrophic culture of Isochrysis globosa 3011 in the dark,not only can obtain a large number of algae species,but also can be stored for a short period of time,and there is still a certain amount of cell viability after recovery,which not only saves time,but also Save cost and occupy space.